Bio-Techne (TECH)

The invention provides methods for detecting target double stranded nucleic acids, including for in situ hybridization assays.

A surface plasmon resonance chip includes a sensor surface having a substrate, an inert metal layer directly on the substrate, a surface layer directly on the inert metal layer, and fusion proteins immobilized on the surface layer.

The invention relates to methods of multiplex detection of a plurality of target nucleic acids using combinations of labels by contacting a sample comprising a cell comprising a plurality of target nucleic acids with a set of probes, wherein the set of probes comprises subsets of probes comprising a plurality detectable labels that provide unique labeling of each target nucleic acid, wherein each probe subset comprises one or more distinct labels, wherein the number and/or combination of distinct labels is unique for each target nucleic acid; and detecting the detectable labels bound to the respective target nucleic acids.

The invention relates to methods of in situ detection of a nucleic acid variation of a target nucleic acid in a sample, including single nucleotide variations, multi-nucleotide variations or splice sites.

This disclosure describes isolated protein complexes including a Wnt and a sFRP; compositions including a Wnt; a cell overexpressing a Wnt and a sFRP; compositions including a cell overexpressing a Wnt and a cell overexpressing a sFRP; methods of making the protein complexes, compositions, and cells; and methods of using the isolated protein complexes, compositions, and cells.