UNITED STATES

SECURITIES AND EXCHANGE COMMISSION

Washington, D.C. 20549

FORM 10-Q

(Mark One)

☒ Quarterly Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934

For the quarterly period ended ~~March 31,~~June 30, 2022

☐ Transition Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934

For the transition period from _____________ to _____________

Commission File Number 001-38185

PRESSURE BIOSCIENCES, INC.

(Exact name of registrant as specified in its charter)

Massachusetts		04-2652826
(State or other jurisdiction of		(I.R.S. Employer
incorporation or organization)		Identification No.)

14 Norfolk Avenue

South Easton, Massachusetts

02375

(Address of principal executive offices)

(Zip Code)

(508) 230-1828

(Registrant’s telephone number, including area code)

Securities registered pursuant to Section 12(b) of the Act:

Title of each class		Trading Symbol(s)		Name of each exchange on which registered
N/A		N/A		N/A

Indicate by check mark whether the registrant (1) has filed all reports required to be filed by Section 13 or 15(d) of the Securities Exchange Act of 1934 during the preceding 12 months (or for such shorter period that the registrant was required to file such reports), and (2) has been subject to such filing requirements for the past 90 days.

☒ Yes ☐ No

Indicate by check mark whether the registrant has submitted electronically every Interactive Data File required to be submitted pursuant to Rule 405 of Regulation S-T (§232.405 of this chapter) during the preceding 12 months (or for such shorter period that the registrant was required to submit such files).

☒ Yes ☐ No

Indicate by check mark whether the registrant is a large accelerated filer, an accelerated filer, a non-accelerated filer, or a smaller reporting company. See the definitions of “large accelerated filer,” “accelerated filer,” “smaller reporting company,” and “emerging growth company” in Rule 12b-2 of the Exchange Act. (Check one):

Large accelerated filer	☐	Accelerated filer	☐
Non-accelerated Filer	☒	Smaller Reporting Company	☒
Emerging Growth Company	☐

If an emerging growth company, indicate by check mark if the registrant has elected not to use the extended transition period for complying with any new or revised financial accounting standards provided pursuant to Section 13(a) of the Exchange Act. ☐

Indicate by check mark whether the registrant is a shell company (as defined in Exchange Act Rule 12b-2 of the Exchange Act).

☐ Yes ☒ No

The number of shares outstanding of the Issuer’s common stock as of ~~May 13,~~August 8, 2022 was ~~8,788,098~~9,074,069.

TABLE OF CONTENTS

	Page

PART I - FINANCIAL INFORMATION	3

Item 1. Unaudited Financial Statements	3

Consolidated Balance Sheets as of ~~March 31,~~June 30, 2022 and December 31, 2021	3

Consolidated Statements of Operations for the Three and Six months Ended ~~March 31,~~June 30, 2022 and 2021	4

Consolidated Statements of Cash Flows for the ~~Three~~Six Months Ended ~~March 31,~~June 30, 2022 and 2021	5

Consolidated Statements of Changes in Stockholders’ Deficit for the Three and Six Months Ended ~~March 31,~~June 30, 2022 and 2021	6

Notes to Unaudited Consolidated Financial Statements	8

Item 2. Management’s Discussion and Analysis of Financial Condition and Results of Operations	2621

Item 3. Quantitative and Qualitative Disclosure About Market Risk	3024

Item 4. Controls and Procedures	3024

PART II - OTHER INFORMATION	3125

Item 1. Legal Proceedings	3125

Item 1A. Risk Factors	3125

Item 2. Unregistered Sales of Equity Securities and Use of Proceeds	3226

Item 3. Defaults Upon Senior Securities	3226

Item 4. Mine Safety Disclosures	3226

Item 5. Other Information	3226

Item 6. Exhibits	3326

SIGNATURES	3427

PART I - FINANCIAL INFORMATION

Item 1. Financial Statements

PRESSURE BIOSCIENCES, INC. AND SUBSIDIARIES

CONSOLIDATED BALANCE SHEETS

(UNAUDITED)


	March 31, 2022			December 31, 2021			June 30, 2022			December 31, 2021
ASSETS
CURRENT ASSETS
Cash and cash equivalents	$	118,803		$	132,311		$	121,569		$	132,311
Accounts receivable		291,846			154,746			316,796			154,746
Inventories, net of $342,496 reserve at March 31, 2022 and December 31, 2021		1,360,539			1,147,554
Inventories, net of $342,496 reserve at June 30, 2022 and December 31, 2021								1,355,676			1,147,554
Prepaid expenses and other current assets		184,936			422,617			228,254			422,617
Total current assets		1,956,124			1,857,228			2,022,295			1,857,228
Investment in equity securities		79,114			59,976			60,604			59,976
Property and equipment, net		103,520			115,846			101,798			115,846
Right of use asset leases		369,006			395,565			341,681			395,565
Intangible assets, net		382,211			403,846			360,577			403,846
TOTAL ASSETS	$	2,889,975		$	2,832,461		$	2,886,955		$	2,832,461
LIABILITIES AND STOCKHOLDERS’ DEFICIT
CURRENT LIABILITIES
Accounts payable	$	665,228		$	527,924		$	552,691		$	527,924
Accrued employee compensation		170,212			117,680			237,102			117,680
Accrued professional fees and other		2,133,392			1,955,672			2,157,575			1,955,672
Other current liabilities		8,304,648			7,757,217			9,036,299			7,757,217
Deferred revenue		45,806			37,124			31,851			37,124
Convertible debt, net of unamortized discounts of $813,478 and $1,536,649, respectively		14,290,657			12,839,813
Other debt, net of unamortized discounts of $11,670 and $0, respectively		1,311,495			1,256,840
Convertible debt, net of unamortized discounts of $381,223 and $1,536,649, respectively								15,686,451			12,839,813
Other debt, net of unamortized discounts of $16,900 and $0, respectively								1,621,854			1,256,840
Operating lease liability		135,810			132,996			138,691			132,996
Other related party debt		195,250			-
Other related party debt, net of unamortized discounts of $23,646 and $0, respectively								283,804			-
Total current liabilities		27,252,498			24,625,266			29,746,318			24,625,266
LONG TERM LIABILITIES
Long term debt		150,000			150,000			150,000			150,000
Operating lease liability – long term		233,196			262,569			202,990			262,569
Deferred revenue		2,592			3,587			-			3,587
TOTAL LIABILITIES		27,638,286			25,041,422			30,099,308			25,041,422
COMMITMENTS AND CONTINGENCIES (Note 4)		-			-			-			-
STOCKHOLDERS’ DEFICIT
Series D Convertible Preferred Stock, $.01 par value; 850 shares authorized; 300 shares issued and outstanding on March 31, 2022 and December 31, 2021, respectively (Liquidation value of $300,000)		3			3
Series G Convertible Preferred Stock, $.01 par value; 240,000 shares authorized; 80,570 shares issued and outstanding on March 31, 2022 and December 31, 2021, respectively		806			806
Series H Convertible Preferred Stock, $.01 par value; 10,000 shares authorized; 10,000 shares issued and outstanding on March 31, 2022 and December 31, 2021, respectively		100			100
Series H2 Convertible Preferred Stock, $.01 par value; 21 shares authorized; 21 shares issued and outstanding on March 31, 2022 and December 31, 2021, respectively		-			-
Series J Convertible Preferred Stock, $.01 par value; 6,250 shares authorized; 3,458 shares issued and outstanding on March 31, 2022 and December 31, 2021, respectively		35			35
Series K Convertible Preferred Stock, $.01 par value; 15,000 shares authorized; 6,880 shares issued and outstanding on March 31, 2022 and December 31, 2021, respectively		68			68
Series AA Convertible Preferred Stock, $.01 par value; 10,000 shares authorized; 8,649 shares issued and outstanding on March 31, 2022 and December 31, 2021, respectively		87			87
Series D Convertible Preferred Stock, $.01 par value; 850 shares authorized; 300 shares issued and outstanding on June 30, 2022 and December 31, 2021, respectively (Liquidation value of $300,000)								3			3
Series G Convertible Preferred Stock, $.01 par value; 240,000 shares authorized; 80,570 shares issued and outstanding on June 30, 2022 and December 31, 2021, respectively								806			806
Series H Convertible Preferred Stock, $.01 par value; 10,000 shares authorized; 10,000 shares issued and outstanding on June 30, 2022 and December 31, 2021, respectively								100			100
Series H2 Convertible Preferred Stock, $.01 par value; 21 shares authorized; 21 shares issued and outstanding on June 30, 2022 and December 31, 2021, respectively								-			-
Series J Convertible Preferred Stock, $.01 par value; 6,250 shares authorized; 3,458 shares issued and outstanding on June 30, 2022 and December 31, 2021, respectively								35			35
Series K Convertible Preferred Stock, $.01 par value; 15,000 shares authorized; 6,880 shares issued and outstanding on June 30, 2022 and December 31, 2021, respectively								68			68
Series AA Convertible Preferred Stock, $.01 par value; 10,000 shares authorized; 8,645 and 8,649 shares issued and outstanding on June 30, 2022 and December 31, 2021, respectively								86			87
Preferred Stock		87			87
Common stock, $.01 par value; 100,000,000 shares authorized; 10,194,136 and 9,120,526 shares issued and outstanding on March 31, 2022 and December 31, 2021 respectively		101,942			91,206
Common stock, $.01 par value; 100,000,000 shares authorized; 10,703,179 and 9,120,526 shares issued and outstanding on June 30, 2022 and December 31, 2021, respectively								107,033			91,206
Warrants to acquire common stock		31,974,888			31,715,154			31,974,888			31,715,154
Additional paid-in capital		63,867,846			64,261,048			64,746,760			64,261,048
Accumulated deficit		(120,694,086	)		(118,277,468	)		(124,042,132	)		(118,277,468	)
Total stockholders’ deficit		(24,748,311	)		(22,208,961	)		(27,212,353	)		(22,208,961	)
TOTAL LIABILITIES AND STOCKHOLDERS’ DEFICIT	$	2,889,975		$	2,832,461		$	2,886,955		$	2,832,461

The accompanying notes are an integral part of these unaudited consolidated financial statements

PRESSURE BIOSCIENCES, INC. AND SUBSIDIARIES

CONSOLIDATED STATEMENTS OF OPERATIONS

(UNAUDITED)

	2022			2021
	For the Three Months Ended March 31,						For the Three Months Ended June 30,						For the Six Months Ended June 30,
	2022			2021			2022			2021			2022			2021
Revenue:
Products, services, other	$	480,000		$	559,874		$	498,137		$	608,927		$	978,137		$	1,168,801
Total revenue		480,000			559,874			498,137			608,927			978,137			1,168,801

Costs and expenses:
Cost of products and services		314,363			226,275			302,141			286,660			616,504			512,935
Research and development		281,589			299,943			172,726			256,507			454,315			556,450
Selling and marketing		66,462			93,328			129,434			92,813			195,896			186,141
General and administrative		903,885			1,015,430			795,466			619,286			1,699,351			1,634,716
Total operating costs and expenses		1,566,299			1,634,976			1,399,767			1,255,266			2,966,066			2,890,242

Operating loss		(1,086,299	)		(1,075,102	)		(901,630	)		(646,339	)		(1,987,929	)		(1,721,441	)

Other (expense) income:
Interest expense, net		(2,579,161	)		(4,668,064	)		(1,835,589	)		(3,526,141	)		(4,414,750	)		(8,194,205	)
Unrealized (loss) gain on investment in equity securities		19,138			(107,903	)		(18,510	)		(134,477	)		628			(242,380	)
Loss on extinguishment of liabilities		(589,850	)		(725,159	)		(165,277	)		(498,226	)		(755,127	)		(1,223,385	)
Other income (expense)		(3,513	)		(1,359	)
Other income								4,668			60,012			1,155			58,653
Total other expense		(3,153,386	)		(5,502,485	)		(2,014,708	)		(4,098,832	)		(5,168,094	)		(9,601,317	)

Net loss		(4,239,685	)		(6,577,587	)		(2,916,338	)		(4,745,171	)		(7,156,023	)		(11,322,758	)
Deemed dividends on beneficial conversion feature		-			(57,884	)		-			-			-			(57,884	)
Preferred stock dividends		(432,149	)		(403,215	)		(431,708	)		(404,171	)		(863,857	)		(807,386	)
Net loss attributable to common stockholders	$	(4,671,834	)	$	(7,038,686	)	$	(3,348,046	)	$	(5,149,342	)	$	(8,019,880	)	$	(12,188,028	)
Basic and diluted net loss per share attributable to common stockholders	$	(0.48	)	$	(1.45	)	$	(0.32	)	$	(0.90	)	$	(0.80	)	$	(2.29	)

Weighted average common stock shares outstanding used in the basic and diluted net loss per share calculation		9,695,189			4,865,826			10,462,520			5,748,711			10,029,068			5,312,172

The accompanying notes are an integral part of these unaudited consolidated financial statements

PRESSURE BIOSCIENCES, INC. AND SUBSIDIARIES

CONSOLIDATED STATEMENTS OF CASH FLOWS

(UNAUDITED)

	2022			2021				1			2
	For the Three Months Ended						For the Six Months Ended
	March 31,						June 30,
	2022			2021			2022			2021
CASH FLOWS FROM OPERATING ACTIVITIES:
Net loss	$	(4,239,685	)	$	(6,577,587	)	$	(7,156,023	)	$	(11,322,758	)
Adjustments to reconcile net loss to net cash used in operating activities:
Gain on loan forgiveness		(10,000	)		(367,039	)		(10,000	)		(367,039	)
Non-cash lease expense		26,559			15,576			53,884			31,624
Common stock and warrants issued for interest and extension fees		1,173,458			2,622,078			1,561,973			4,054,749
Depreciation and amortization		35,096			27,190			62,207			56,291
Accretion of interest and amortization of debt discount		817,877			2,165,780			1,457,204			3,909,024
Loss on extinguishment of accrued liabilities and debt		589,570			-			755,127			-
Stock-based compensation expense		64,483			61,237			96,557			124,695
(Gain) loss on investment in equity securities		(19,138	)		107,903			(628	)		242,380
Common stock and warrants issued for services		117,461			238,512			185,261			238,512
Changes in operating assets and liabilities:
Accounts receivable		(137,100	)		(373,268	)		(162,050	)		(498,538	)
Inventories		(212,985	)		33,402			(208,122	)		100,672
Prepaid expenses and other assets		237,681			43,291			194,363			95,775
Accounts payable		137,304			(60,613	)		24,767			(143,110	)
Accrued employee compensation		52,532			42,702			119,422			2,932
Operating lease liability		(26,559	)		(15,576	)		(53,884	)		(31,624	)
Deferred revenue and other accrued expenses		637,164			799,397			1,081,750			1,415,688
Net cash used in operating activities		(756,282	)		(1,237,015	)		(1,998,192	)		(2,090,727	)

CASH FLOWS FROM INVESTING ACTIVITIES:
Purchases of property plant and equipment		(1,135	)		(3,962	)		(4,890	)		(3,962	)
Net cash used in investing activities		(1,135	)		(3,962	)		(4,890	)		(3,962	)

CASH FLOWS FROM FINANCING ACTIVITIES:
Net proceeds from Series AA Convertible Preferred Stock		-			100,000			-			100,000
Proceeds from stock option exercises		-			14,773			17,443			14,773
Net proceeds from convertible debt		806,000			730,000			2,209,750			2,598,250
Net proceeds from non-convertible debt – third party		351,150			854,538			1,288,100			1,183,188
Net proceeds from non-convertible debt – related party		195,250			85,000			464,500			171,600
Payments on convertible debt		(324,350	)		(191,250	)		(865,367	)		(1,200,996	)
Payments on non-convertible debt – related party		-			(70,000	)		(209,000	)		(153,000	)
Payments on non-convertible debt		(284,141	)		(250,094	)
Payments on non-convertible debt – third party								(913,086	)	`	(591,041	)
Net cash provided by financing activities		743,909			1,272,967			1,992,340			2,122,774

NET INCREASE IN CASH AND CASH EQUIVALENTS		(13,508	)		31,990
NET INCREASE (DECREASE) IN CASH AND CASH EQUIVALENTS								(10,742	)		28,085
CASH AND CASH EQUIVALENTS AT BEGINNING OF YEAR		132,311			18,540			132,311			18,540
CASH AND CASH EQUIVALENTS AT END OF PERIOD	$	118,803		$	50,530		$	121,569		$	46,625

SUPPLEMENTAL INFORMATION
Interest paid in cash	$	120,436		$	37,375		$	515,210		$	383,403
NON CASH TRANSACTIONS:
Common stock issued with debt		142,480			-			178,328			112,877
Discount from warrants issued with debt		87,436			162,654			87,436			1,068,842
Common stock issued in lieu of cash for dividend		64,256			-			215,277			114,298
Early adoption of ASU 2020-06		473,027			-			473,027			-
Preferred stock dividends		432,149			403,215			863,857			807,386
Conversion of debt and interest into common stock		350,500			118,000			350,500			349,350
Discount due to beneficial conversion feature		-			53,777			-			566,847
Deemed dividend - beneficial conversion feature		-			57,884			-			57,884
Conversion of preferred stock for common stock								44			-

The accompanying notes are an integral part of these unaudited consolidated financial statements

PRESSURE BIOSCIENCES, INC. AND SUBSIDIARIES

CONSOLIDATED STATEMENTS OF CHANGES IN STOCKHOLDERS’ DEFICIT

(UNAUDITED)

Shares Amount Shares Amount Shares Amount Shares Amount Shares Amount Shares Amount Shares Amount Shares Amount Warrants Capital Deficit Deficit
Series D
Preferred Stock Series G
Preferred Stock Series H
Preferred Stock Series H(2)
Preferred Stock Series J
Preferred Stock Series K
Preferred Stock Series AA
Preferred Stock Common Stock Stock Additional Paid-In Accumulated Total
Stockholders’
Shares Amount Shares Amount Shares Amount Shares Amount Shares Amount Shares Amount Shares Amount Shares Amount Warrants Capital Deficit Deficit
BALANCE, December 31, 2021 300 $ 3 80,570 $ 806 10,000 $ 100 21 $ - 3,458 $ 35 6,880 $ 68 8,649 $ 87 9,120,526 $ 91,206 $ 31,715,154 $ 64,261,048 $ (118,277,468 ) $ (22,208,961 )
Early adoption of ASU 2020-06 - - - - - - - - - - - - - - - - - (2,728,243 ) 2,255,216 (473,027 )
Stock-based compensation - - - - - - - - - - - - - - - - - 64,483 - 64,483
Series AA Preferred Stock dividend - - - - - - - - - - - - - - - - - - (432,149 ) (432,149 )
Series AA Preferred Stock offering
Series AA Preferred Stock offering, shares
Beneficial conversion option on convertible preferred stock
Issuance of common stock for services - - - - - - - - - - - - - - 37,000 370 - 77,330 - 77,700
Issuance of common stock warrants for services - - - - - - - - - - - - - - - - 39,761 - - 39,761
Warrants issued for debt extension - - - - - - - - - - - - - - - - 132,537 - - 132,537
Common stock issued for debt extension - - - - - - - - - - - - - - 214,500 2,145 - 470,755 - 472,900
Conversion of debt and interest for common stock - - - - - - - - - - - - - - 140,200 1,402 - 349,098 - 350,500
Issuance of common stock for dividends paid-in-kind - - - - - - - - - - - - - - 31,810 318 - 63,938 - 64,256
Issuance of common stock for interest paid-in-kind - - - - - - - - - - - - - - 558,100 5,581 - 1,167,877 - 1,173,458
Stock issued with debt - - - - - - - - - - - - - - 92,000 920 - 141,560 - 142,480
Warrants issued with debt - - - - - - - - - - - - - - - - 87,436 - - 87,436
Net loss - - - - - - - - - - - - - - - - - - (4,239,685 ) (4,239,685 )
BALANCE, March 31, 2022 300 $ 3 80,570 $ 806 10,000 $ 100 21 $ - 3,458 $ 35 6,880 $ 68 8,649 $ 87 10,194,136 $ 101,942 $ 31,974,888 $ 63,867,846 $ (120,694,086 ) $ (24,748,311 )

				1				2				3				4				5				6					7					8		9		10			11			12
	Series D				Series G				Series H				Series H(2)				Series J				Series K				Series AA												Additional						Total
	Preferred Stock				Preferred Stock				Preferred Stock				Preferred Stock				Preferred Stock				Preferred Stock				Preferred Stock						Common Stock				Stock		Paid-In			Accumulated			Stockholders’
	Shares		Amount		Shares		Amount		Shares		Amount		Shares		Amount		Shares		Amount		Shares		Amount		Shares			Amount			Shares		Amount		Warrants		Capital			Deficit			Deficit
BALANCE, December 31, 2021		300	$	3		80,570	$	806		10,000	$	100		21	$	-		3,458	$	35		6,880	$	68		8,649		$	87			9,120,526	$	91,206	$	31,715,154	$	64,261,048		$	(118,277,468	)	$	(22,208,961	)
Early adoption of ASU 2020-06		-		-		-		-		-		-		-		-		-		-		-		-		-			-			-		-		-		(2,728,243	)		2,255,216			(473,027	)
Stock-based compensation		-		-		-		-		-		-		-		-		-		-		-		-		-			-			-		-		-		64,483			-			64,483
Series AA Preferred Stock dividend		-		-		-		-		-		-		-		-		-		-		-		-		-			-			-		-		-		-			(432,149	)		(432,149	)
Issuance of common stock for services		-		-		-		-		-		-		-		-		-		-		-		-		-			-			37,000		370		-		77,330			-			77,700
Issuance of common stock warrants for services		-		-		-		-		-		-		-		-		-		-		-		-		-			-			-		-		39,761		-			-			39,761
Warrants issued for debt extension		-		-		-		-		-		-		-		-		-		-		-		-		-			-			-		-		132,537		-			-			132,537
Common stock issued for debt extension		-		-		-		-		-		-		-		-		-		-		-		-		-			-			214,500		2,145		-		470,755			-			472,900
Conversion of debt and interest for common stock		-		-		-		-		-		-		-		-		-		-		-		-		-			-			140,200		1,402		-		349,098			-			350,500
Issuance of common stock for dividends paid-in-kind		-		-		-		-		-		-		-		-		-		-		-		-		-			-			31,810		318		-		63,938			-			64,256
Issuance of common stock for interest paid-in-kind		-		-		-		-		-		-		-		-		-		-		-		-		-			-			558,100		5,581		-		1,167,877			-			1,173,458
Stock issued with debt		-		-		-		-		-		-		-		-		-		-		-		-		-			-			92,000		920		-		141,560			-			142,480
Warrants issued with debt		-		-		-		-		-		-		-		-		-		-		-		-		-			-			-		-		87,436		-			-			87,436
Net loss		-		-		-		-		-		-		-		-		-		-		-		-		-			-			-		-		-		-			(4,239,685	)		(4,239,685	)
BALANCE, March 31, 2022		300	$	3		80,570	$	806		10,000	$	100		21	$	-		3,458	$	35		6,880	$	68		8,649		$	87			10,194,136	$	101,942	$	31,974,888	$	63,867,846		$	(120,694,086	)	$	(24,748,311	)
Stock-based compensation		-		-		-		-		-		-		-		-		-		-		-		-		-			-			-		-		-		32,074			-			32,074
Stock option exercise		-		-		-		-		-		-		-		-		-		-		-		-		-			-			25,279		253		-		17,190			-			17,443
Series AA Preferred Stock dividend		-		-		-		-		-		-		-		-		-		-		-		-		-			-			-		-		-		-			(431,708	)		(431,708	)
Issuance of common stock for services		-		-		-		-		-		-		-		-		-		-		-		-		-			-			40,000		400		-		67,400			-			67,800
Common stock issued for debt extension		-		-		-		-		-		-		-		-		-		-		-		-		-			-			106,400		1,064		-		190,239			-			191,303
Conversion of preferred stock for common stock		-		-		-		-		-		-		-		-		-		-		-		-		(4	)		(1	)		4,400		44		-		(43	)		-			-
Issuance of common stock for dividends paid-in-kind		-		-		-		-		-		-		-		-		-		-		-		-		-			-			86,464		865		-		150,156			-			151,021
Issuance of common stock for interest paid-in-kind		-		-		-		-		-		-		-		-		-		-		-		-		-			-			224,500		2,245		-		386,270			-			388,515
Stock issued with debt		-		-		-		-		-		-		-		-		-		-		-		-		-			-			22,000		220		-		35,628			-			35,848
Net loss		-		-		-		-		-		-		-		-		-		-		-		-		-			-			-		-		-		-			(2,916,338	)		(2,916,338	)
BALANCE, June 30, 2022		300	$	3		80,570	$	806		10,000	$	100		21	$	-		3,458	$	35		6,880	$	68		8,645		$	86			10,703,179	$	107,033	$	31,974,888	$	64,746,760		$	(124,042,132	)	$	(27,212,353	)

Series D
Preferred Stock Series G
Preferred Stock Series H
Preferred Stock Series H(2)
Preferred Stock Series J
Preferred Stock Series K
Preferred Stock Series AA
Preferred Stock Common Stock Stock Additional Paid-In Accumulated Total
Stockholders’
Shares Amount Shares Amount Shares Amount Shares Amount Shares Amount Shares Amount Shares Amount Shares Amount Warrants Capital Deficit Deficit

BALANCE, December 31, 2020 300 $ 3 80,570 $ 806 10,000 $ 100 21 $ - 3,458 $ 35 6,880 $ 68 8,043 81 4,168,324 $ 41,683 $ 29,192,471 $ 50,312,968 $ (96,465,807 ) $ (16,917,592 )
Stock-based compensation - - - - - - - - - - - - - - - - - 61,237 - 61,237
Stock option exercise - - - - - - - - - - - - - - 21,411 214 - 14,559 - 14,773
Series AA Preferred Stock dividend - - - - - - - - - - - - - - - - - - (403,215 ) (403,215 )
Issuance of warrants for interest paid-in-kind - - - - - - - - - - - - - - - - 600,298 - - 600,298
Issuance of common stock for services - - - - - - - - - - - - - - 112,400 1,124 237,388 - 238,512
Beneficial conversion feature on debt - - - - - - - - - - - - - - - - - 53,777 - 53,777
Series AA Preferred Stock offering - - - - - - - - - - - - 40 - - - 49,884 50,116 - 100,000
Beneficial conversion option on convertible preferred stock - - - - - - - - - - - - - - - - - 57,884 - 57,884
Deemed dividend on convertible preferred stock - - - - - - - - - - - - - - - - - (57,884 ) - (57,884 )
Conversion of debt and interest for common stock - - - - - - - - - - - - - - 47,200 472 - 117,528 - 118,000
Issuance of common stock for interest paid in kind - - - - - - - - - - - - - - 922,372 9,224 - 2,012,556 - 2,021,780
Warrants issued with debt - - - - - - - - - - - - - - - - 162,654 - - 162,654
Net loss - - - - - - - - - - - - - - - - - - (6,577,587 ) (6,577,587 )
BALANCE, March 31, 2021 300 $ 3 80,570 $ 806 10,000 $ 100 21 $ - 3,458 $ 35 6,880 $ 68 8,083 $ 81 5,271,707 $ 52,717 $ 30,005,307 $ 52,860,129 $ (103,446,609 ) $ (20,527,363 )

	Series D Preferred Stock				Series G Preferred Stock				Series H Preferred Stock				Series H(2) Preferred Stock				Series J Preferred Stock				Series K Preferred Stock				Series AA Preferred Stock				Common Stock				Stock		Additional Paid-In			Accumulated			Total Stockholders’
	Shares		Amount		Shares		Amount		Shares		Amount		Shares		Amount		Shares		Amount		Shares		Amount		Shares		Amount		Shares		Amount		Warrants		Capital			Deficit			Deficit

BALANCE, December 31, 2020		300	$	3		80,570	$	806		10,000	$	100		21	$	-		3,458	$	35		6,880	$	68		8,043		81		4,168,324	$	41,683	$	29,192,471	$	50,312,968		$	(96,465,807	)	$	(16,917,592	)
Stock-based compensation		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		61,237			-			61,237
Stock option exercise		-		-		-		-		-		-		-		-		-		-		-		-		-		-		21,411		214		-		14,559			-			14,773
Series AA Preferred Stock dividend		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-			(403,215	)		(403,215	)
Issuance of warrants for interest paid-in-kind		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		600,298		-			-			600,298
Issuance of common stock for services		-		-		-		-		-		-		-		-		-		-		-		-		-		-		112,400		1,124		-		237,388			-			238,512
Beneficial conversion feature on debt		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		53,777			-			53,777
Series AA Preferred Stock offering		-		-		-		-		-		-		-		-		-		-		-		-		40		-		-		-		49,884		50,116			-			100,000
Beneficial conversion option on convertible preferred stock		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		57,884			-			57,884
Deemed dividend on convertible preferred stock		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		(57,884	)		-			(57,884	)
Conversion of debt and interest for common stock		-		-		-		-		-		-		-		-		-		-		-		-		-		-		47,200		472		-		117,528			-			118,000
Issuance of common stock for interest paid in kind		-		-		-		-		-		-		-		-		-		-		-		-		-		-		922,372		9,224		-		2,012,556			-			2,021,780
Warrants issued with debt		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		162,654		-			-			162,654
Net loss		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-			(6,577,587	)		(6,577,587	)
BALANCE, March 31, 2021		300	$	3		80,570	$	806		10,000	$	100		21	$	-		3,458	$	35		6,880	$	68		8,083	$	81		5,271,707	$	52,717	$	30,005,307	$	52,860,129		$	(103,446,609	)	$	(20,527,363	)
Stock-based compensation		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		63,458			-			63,458
Series AA Preferred Stock dividend		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-			(404,171	)		(404,171	)
Beneficial conversion feature on debt		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		513,070			-			513,070
Issuance of common stock for interest paid in kind		-		-		-		-		-		-		-		-		-		-		-		-		-		-		720,610		7,206		-		1,425,465			-			1,432,671
Issuance of common stock for dividends paid-in-kind		-		-		-		-		-		-		-		-		-		-		-		-		-		-		56,067		560		-		113,738			-			114,298
Conversion of debt and interest for common stock		-		-		-		-		-		-		-		-		-		-		-		-		-		-		92,500		925		-		230,325			-			231,250
Stock issued with debt		-		-		-		-		-		-		-		-		-		-		-		-		-		-		120,000		1,200		-		111,677			-			112,877
Warrants issued with debt		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		906,188		-			-			906,188
Net loss		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-		-			(4,745,171	)		(4,745,171	)
BALANCE, June 30, 2021		300	$	3		80,570	$	806		10,000	$	100		21	$	-		3,458	$	35		6,880	$	68		8,083	$	81		6,260,884	$	62,608	$	30,911,495	$	55,317,862		$	(108,595,951	)	$	(22,302,893	)

The accompanying notes are an integral part of these unaudited consolidated financial statements

PRESSURE BIOSCIENCES, INC. AND SUBSIDIARIES

NOTES TO CONSOLIDATED FINANCIAL STATEMENTS

~~MARCH 31,~~JUNE 30, 2022

(UNAUDITED)

1)Business Overview, Liquidity and Management Plans

Pressure Biosciences, Inc. (“we”, “our”, “the Company”) develops and sells innovative, broadly enabling, high pressure-based platform technologies and related consumables for the worldwide life sciences, agriculture, food and beverage, and other key industries. Our solutions are based on the unique properties ~~of both~~ and/or force generated from eitherconstant (i.e., static) ~~and~~or alternating (i.e., ~~pressure cycling technology,~~Pressure Cycling Technology™, or ~~“PCT”~~“PCT™”) hydrostatic pressure. PCT is a patented enabling technology platform that uses alternating cycles of hydrostatic pressure between ambient and ultra-high levels to safely and reproducibly control bio-molecular interactions (e.g., cell lysis, biomolecule extraction). Historically, our primary focus has been inIn the development of PCT-based products for biomarker and target discovery, drug design and development, biotherapeutics characterization and quality control, soil & plant biology, forensics, and counter-bioterror applications. In more recentpast five years, major new market opportunities have emerged in the use of our pressure-based technologies ~~in the following areas:~~in: (1) the use of our recently acquired, patented technology from BaroFold, Inc. (the ~~“BaroFold”~~“BaroFold™” technology) to allow entry into the bio-pharma contract services sector, and (2) the use of our recently-patented, scalable, high-efficiency, pressure-based Ultra Shear ~~Technology~~Technology™ (“~~UST”~~UST™”) platform to (i) create stable nanoemulsions of otherwise immiscible fluids (e.g., oils and water) and to (ii) prepare higher quality, homogenized, extended shelf-life or room temperature stable low-acid liquid foods that cannot be effectively preserved using existing non-thermal technologies. The Company’s initial growth and strong scientific reputation has been generated from PCT, a patented enabling technology platform that uses alternating cycles of hydrostatic pressure between ambient and ultra-high levels to safely and reproducibly control bio-molecular interactions (e.g., cell lysis, biomolecule extraction). While now focused predominantly on the enormous potential and markets for UST, and secondarily BaroFold, our historical concentration was in the development of PCT-based products for biomarker and target discovery, drug design and development, biotherapeutics characterization and quality control, soil & plant biology, forensics, and counter-bioterror applications.

On February 8, 2021, PBI announced plans to acquire the assets of a global eco-friendly agrochemical supplier. On April 14, 2021, PBI finalized terms and executed a new letter of intent to purchase the assets of the agrochemical supplier. This opportunity ~~is attractive as it has~~offered the potential of ~~readily~~ producing significant revenue, as well as the potential to apply the UST technology to improve some of the product line. In July 2021, a newly-formed subsidiary of PBI, PBI Agrochem, leased a warehouse in Carson City, NV, and hired a warehouse manager.

2)Going Concern

The accompanying financial statements have been prepared assuming that the Company will continue as a going concern, which contemplates the realization of assets and the liquidation of liabilities in the normal course of business. However, we have experienced losses from operations and negative cash flows from operations with respect to our pressure cycling technology business since our inception. As of ~~March 31,~~June 30, 2022, we do not have adequate working capital resources to satisfy our current liabilities and as a result, there is substantial doubt regarding our ability to continue as a going concern. We have been successful in raising debt and equity capital in the past and as described in Notes 5 and 6. In addition we raised debt and equity capital after ~~March 31,~~June 30, 2022 as described in Note 7. We have financing efforts in place to continue to raise cash through debt and equity offerings. Although we have successfully completed financings and reduced expenses in the past, we cannot assure you that our plans to address these matters in the future will be successful. These financial statements do not include any adjustments that might result from this uncertainty.

3)Summary of Significant Accounting Policies

Basis of Presentation

The unaudited interim financial statements of Pressure BioSciences, Inc. and its consolidated subsidiaries (collectively, the “Company”) included herein have been prepared by the Company in accordance with the instructions to Form 10-Q and the rules and regulations of the U.S. Securities and Exchange Commission. Under these rules and regulations, some information and footnote disclosures normally included in financial statements prepared under accounting principles generally accepted in the United States of America have been shortened or omitted. Management believes that all adjustments necessary for a fair statement of the financial position and the results of operations for the periods shown have been made. All adjustments are normal and recurring. These financial statements should be read together with the Company’s audited financial statements included in its Form 10-K for the fiscal year ended December 31, 2021. Operating results for the ~~three~~six months ended ~~March 31,~~June 30, 2022 are not necessarily indicative of the final results that may be expected for the year ending December 31, 2022.

Use of Estimates

The Company’s consolidated financial statements and accompanying notes are prepared in accordance with accounting principles generally accepted in the United States of America, which require the use of estimates, judgements and assumptions that affect the reported amounts of assets and liabilities at the date of the financial statements and the reported amounts of revenues and expenses during the periods presented. Global concerns about the COVID-19 pandemic have adversely affected, and we expect will continue to adversely affect, our business, financial condition and results of operations including the estimates and assumptions made by management. Significant estimates and assumptions include valuations of share-based awards, investments in equity securities and intangible asset impairment. Actual results could differ from the estimates, and such differences may be material to the Company’s consolidated financial statements.

Recent Accounting Pronouncements

In August 2020, the Financial Accounting Standards Board (FASB) issued Accounting Standards Update (ASU) No. 2020-06, Accounting for Convertible Instruments and Contracts in an Entity’s Own Equity, as part of its overall simplification initiative to reduce costs and complexity of applying accounting standards while maintaining or improving the usefulness of the information provided to users of financial statements. Among other changes, the new guidance removes the beneficial conversion separation model for convertible debt. As a result, after adopting the guidance, entities will no longer account for beneficial conversion features in equity. The guidance is effective for public business entities, other than small reporting companies financial statements starting January 1, 2022, with early adoption permitted. The Company is a small reporting company and early adopted the new guidance on January 1, 2022 using the modified retrospective approach and recorded a cumulative effect of adoption equal to a $2,728,243 decrease in additional paid in capital and a $2,255,216 decrease in accumulated deficit. There is no ~~expected~~ material impact to the Company’s statements of operations or cash flows as the result of the adoption of ASU 2020-06.

Principles of Consolidation

The consolidated financial statements include the accounts of Pressure BioSciences, Inc., and its wholly owned subsidiaries PBI BioSeq, ~~Inc~~Inc. and PBI Agrochem, Inc. All intercompany accounts and transactions have been eliminated in consolidation.

Revenue Recognition

We recognize revenue in accordance with FASB ASC 606, Revenue from Contracts with Customers, and ASC 340-40, Other Assets and Deferred Costs—Contracts with Customers. Revenue is measured based on a consideration specified in a contract with a customer, and excludes any sales incentives and amounts collected on behalf of third parties. We enter into sales contracts that may consist of multiple distinct performance obligations where certain performance obligations of the sales contract are not delivered in one reporting period. We measure and allocate revenue according to ASC 606-10.

We identify a performance obligation as distinct if both the following criteria are true: the customer can benefit from the good or service either on its own or together with other resources that are readily available to the customer and the entity’s promise to transfer the good or service to the customer is separately identifiable from other promises in the contract. Determining the standalone selling price (“SSP”) and allocation of consideration from a contract to the individual performance obligations, and the appropriate timing of revenue recognition, is the result of significant qualitative and quantitative judgments. Management considers a variety of factors such as historical sales, usage rates, costs, and expected margin, which may vary over time depending upon the unique facts and circumstances related to each performance obligation in making these estimates. While changes in the allocation of the SSP between performance obligations will not affect the amount of total revenue recognized for a particular contract, any material changes could impact the timing of revenue recognition, which would have a material effect on our financial position and result of operations. This is because the contract consideration is allocated to each performance obligation, delivered or undelivered, at the inception of the contract based on the SSP of each distinct performance obligation.

Taxes assessed by a governmental authority that are both imposed on and concurrent with a specific revenue-producing transaction, that are collected by the Company from a customer, are excluded from revenue.

Shipping and handling costs associated with outbound freight after control over a product has transferred to a customer are accounted for as a fulfillment cost and are in included in cost of revenues as consistent with treatment in prior periods.

Our current Barocycler® instruments require a basic level of instrumentation expertise to set-up for initial operation. To support a favorable first experience for our customers, upon customer request, and for an additional fee, we will send a highly trained technical representative to the customer site to install Barocyclers® that we sell, lease, or rent through our domestic sales force. The installation process includes uncrating and setting up the instrument, followed by introductory user training. Our sales arrangements do not provide our customers with a right of return. Any shipping costs billed to customers are recognized as revenue.

The majority of our instrument and consumable contracts contain pricing that is based on the market price for the product at the time of delivery. Our obligations to deliver product volumes are typically satisfied and revenue is recognized when control of the product transfers to our customers. Concurrent with the transfer of control, we typically receive the right to payment for the shipped product and the customer has significant risks and rewards of ownership of the product. Payment terms require customers to pay shortly after delivery and do not contain significant financing components.

Revenue from scientific services customers is recognized upon completion of each stage of service as defined in service agreements.

We apply ASC 845, “Accounting for Non-Monetary Transactions”, to account for products and services sold through non-cash transactions based on the fair values of the products and services involved, where such values can be determined. Non-cash exchanges would require revenue to be recognized at recorded cost or carrying value of the assets or services sold if any of the following conditions apply:

	a)	The fair value of the asset or service involved is not determinable.

	b)	The transaction is an exchange of a product or property held for sale in the ordinary course of business for a product or property to be sold in the same line of business to facilitate sales to customers other than the parties to the exchange.

	c)	The transaction lacks commercial substance.

We recognize revenue for non-cash transactions at recorded cost or carrying value of the assets or services sold.

We account for lease agreements of our instruments in accordance with ASC 842, Leases. We record revenue over the life of the lease term, and we record depreciation expense on a straight-line basis over the thirty-six-month estimated useful life of the Barocycler® instrument. The depreciation expense associated with assets under lease agreement is included in the “Cost of PCT products and services” line item in our accompanying consolidated statements of operations. Many of our lease and rental agreements allow the lessee to purchase the instrument at any point during the term of the agreement with partial or full credit for payments previously made. We pay all maintenance costs associated with the instrument during the term of the leases.

Deferred revenue represents amounts received from service contracts for which the related revenues have not been recognized because one or more of the revenue recognition criteria have not been met. Revenue from service contracts is recorded ratably over the length of the contract.

109

Disaggregation of revenue

In the following table, revenue is disaggregated by primary geographical market, major product line, and timing of revenue recognition.

Schedule of Disaggregation of Revenue

In thousands of US dollars ($)	Three Months Ended March 31,				Three Months Ended June 30,				Six Months Ended June 30,
Primary geographical markets	2022		2021		2022		2021		2022		2021
North America	$	318	$	208	$	253	$	477	$	571	$	685
Europe		46		84		2		103		48		187
Asia		116		268		243		29		359		297
	$	480	$	560	$	498	$	609	$	978	$	1,169

						1		2		3		4
	Three Months Ended March 31,				Three Months Ended June 30,				Six Months Ended June 30,
Major products/services lines	2022		2021		2022		2021		2022		2021
Hardware	$	284	$	377	$	247	$	336	$	531	$	713
Consumables		40		102		76		44		116		146
Contract research services		15		6		110		136		125		142
Sample preparation accessories		31		29		21		40		52		69
Agrochem products		83		-
Technical support/extended service contracts		17		24		36		34		53		58
Agrochem Products						-		-		83		-
Shipping and handling		10		19		8		16		18		35
Other		-		3		-		3		-		6
	$	480	$	560
Revenue					$	498	$	609	$	978	$	1,169

						1		2		3		4
	Three Months Ended March 31,				Three Months Ended June 30,				Six Months Ended June 30,
Timing of revenue recognition	2022		2021		2022		2021		2022		2021
Products transferred at a point in time	$	448	$	538	$	352	$	440	$	800	$	969
Services transferred over time		32		22		146		169		178		200
	$	480	$	560
Revenue					$	498	$	609	$	978	$	1,169

Contract balances

Schedule of Contract Balances

Contract						32		41
In thousands of US dollars ($)	March 31, 2022		December 31, 2021		June 30, 2022		December 31, 2021
Receivables, which are included in ‘Accounts Receivable’	$	292	$	155	$	317	$	155
Contract liabilities (deferred revenue)		48		41		32		41

Transaction price allocated to the remaining performance obligations.

The following table includes estimated revenue expected to be recognized in the future related to performance obligations that are unsatisfied (or partially unsatisfied) at the end of the reporting period.

Schedule of Future Related to Performance Obligations

In thousands of US dollars ($)		2022				2023				Total				2022				2023				Total
Extended warranty service		$	46			$	3			$	49			$	32			$	-			$	32

All consideration from contracts with customers is included in the amounts presented above.

Contract Costs

The Company recognizes the incremental costs of obtaining contracts as an expense when incurred if the amortization period of the assets that the Company otherwise would have recognized is one year or less. These costs are included in selling, general, and administrative expenses. The costs to obtain a contract are recorded immediately in the period when the revenue is recognized either upon shipment or installation. The costs to obtain a service contract are considered immaterial when spread over the life of the contract so the Company records the costs immediately upon billing.

1210

Concentrations

Credit Risk

Our financial instruments that potentially subject us to concentrations of credit risk consist primarily of cash, cash equivalents, and trade receivables. We have cash investment policies which, among other things, limit investments to investment-grade securities. We perform ongoing credit evaluations of our customers, and the risk with respect to trade receivables is further mitigated by the fact that many of our customers are government institutions, large pharmaceutical and biotechnology companies, and academic laboratories.

The following table illustrates the level of concentration as a percentage of total revenues during the three and six months ended ~~March 31,~~June 30, 2022 and 2021.

Schedule of Customer Concentration Risk Percentage

For the Three Months Ended
March 31,
2022 2021
Top Five Customers 73 % 88 %
Federal Agencies 0 % 1 %

	For the Three Months Ended						For the Six Months Ended
	June 30,						June 30,
	2022			2021			2022			2021
Top Five Customers		69	%		50	%		61	%		50	%
Federal Agencies		0	%		14	%		0	%		8	%

The following table illustrates the level of concentration as a percentage of net accounts receivable balance as of ~~March 31,~~June 30, 2022 and December 31, 2021. The Top Five Customers category may include federal agency receivable balances if applicable.

	March 31, 2022			December 31, 2021			June 30, 2022			December 31, 2021
Top Five Customers		88	%		82	%		92	%		82	%
Federal Agencies		3	%		5	%		0	%		5	%

Product Supply

In recent years we utilized a contract assembler for our Barocycler® 2320EXT. They provided us with precision manufacturing services that included management support services to meet our specific application and operational requirements. Among the services provided to us were:

	●	CNC Machining

	●	Contract Assembly & Kitting

	●	Component and Subassembly Design

	●	Inventory Management

	●	ISO certification

Beginning in July 2021, we brought the assembly of our Barocycler 2320EXT instruments in-house. This became necessary when our independent contract assembler (CBM Industries) informed us that they were about to need 100% of their assembly space for one of their customers, who was in fact one of the largest life science instrument manufacturers in the U.S. We worked with our notified body to gain approval to use both the CE and CSA marks on the instrument, which we received during Q3 2021. Until further notice, we expect to continue to assemble our Barocycler 2320EXT instrument at our South Easton, MA location.

We currently manufacture and assemble the Barocycler®, HUB440, HUB880, the SHREDDER SG3, and most of our consumables at our South Easton, MA facility. We will regularly reassess the tradeoffs between in-house assembly versus the benefits of outsourced relationships for of the entire Barocycler® product line, and future instruments.

1311

Investment in Equity Securities

As of ~~March 31,~~June 30, 2022, we held 100,250 shares of common stock of Nexity Global SA, (a Polish publicly traded company).

We account for this investment in accordance with ASC 320 “Investments — Debt and Equity Securities”. ASC 320 requires equity investments with readily determinable fair values to be measured at fair value with changes in fair value recognized in net income.

As of ~~March 31,~~June 30, 2022, our consolidated balance sheet reflected the fair value, determined on a recurring basis based on Level 1 inputs of our investment in Nexity, to be $~~79,114~~60,604. We recorded $~~19,138~~628 as an unrealized ~~gain~~gains during the ~~three~~six months ended ~~March 31,~~June 30, 2022 for changes in market value.

Computation of Loss per Share

Basic loss per share is computed by dividing loss available to common shareholders by the weighted average number of common shares outstanding. Diluted loss per share is computed by dividing loss available to common shareholders by the weighted average number of common shares outstanding plus additional common shares that would have been outstanding if dilutive potential common shares had been issued. For purposes of this calculation, convertible preferred stock, common stock dividends, and warrants and options to acquire common stock, are all considered common stock equivalents in periods in which they have a dilutive effect and are excluded from this calculation in periods in which these are anti-dilutive to our net loss.

The following table illustrates our computation of loss per share for the three and six months ended ~~March 31,~~June 30, 2022 and 2021:

Schedule of Computation of Loss Per Share

2022 2021
For the Three Months Ended
March 31,
2022 2021
Numerator:
Net loss attributable to common stockholders $ (4,671,834 ) $ (7,038,686 )

Denominator for basic and diluted loss per share:
Weighted average common stock shares outstanding 9,695,189 4,865,826

Loss per common share – basic and diluted $ (0.48 ) $ (1.45 )


	For the Three Months Ended						For the Six Months Ended
	June 30,						June 30,
	2022			2021			2022			2021
Numerator:
Net loss attributable to common stockholders	$	(3,348,046	)	$	(5,149,342	)	$	(8,019,880	)	$	(12,188,028	)

Denominator for basic and diluted loss per share:
Weighted average common stock shares outstanding		10,462,520			5,748,711			10,029,068			5,312,172

Loss per common share – basic and diluted	$	(0.32	)	$	(0.90	)	$	(0.80	)	$	(2.29	)

The following table presents securities that could potentially dilute basic loss per share in the future. For all periods presented, the potentially dilutive securities were not included in the computation of diluted loss per share because these securities would have been anti-dilutive to our net loss. The Series D Convertible Preferred Stock, Series G Convertible Preferred Stock, Series H and H2 Convertible Preferred Stock, Series J Convertible Preferred Stock, Series K Convertible Preferred Stock, and Series AA Convertible Preferred Stock are presented below as if they were converted into common shares according to the conversion terms.

Schedule of Anti-dilutive Securities Excluded from Computation of Earnings Per Share


	As of March 31,				As of June 30,
	2022		2021		2022		2021
Stock options		1,307,822		1,358,490		1,307,822		1,350,046
Convertible debt		5,668,816		4,757,701		6,102,145		5,083,187
Common stock warrants		16,350,438		14,901,211		16,287,936		15,703,807
Convertible preferred stock:
Series D Convertible Preferred Stock		25,000		25,000		25,000		25,000
Series G Convertible Preferred Stock		26,857		26,857		26,857		26,857
Series H Convertible Preferred Stock		33,334		33,334		33,334		33,334
Series H2 Convertible Preferred Stock		70,000		70,000		70,000		70,000
Series J Convertible Preferred Stock		115,267		115,267		115,267		115,267
Series K Convertible Preferred Stock		229,334		229,334		229,334		229,334
Series AA Convertible Preferred Stock		8,649,000		8,083,000		8,645,000		8,083,000
		32,475,868		29,600,194
Total potentially dilutive shares						32,842,695		30,719,832

Accounting for Stock-Based Compensation Expense

We maintain equity compensation plans under which incentive stock options and non-qualified stock options are granted to employees, independent members of our Board of Directors and outside consultants. We recognize stock-based compensation expense over the requisite service period using the Black-Scholes formula to estimate the fair value of the stock options on the date of grant.

Determining Fair Value of Stock Option Grants

Valuation and Amortization Method - The fair value of each option award is estimated on the date of grant using the Black-Scholes pricing model based on certain assumptions. The estimated fair value of employee stock options is amortized to expense using the straight-line method over the vesting period.

Expected Term - The Company uses the simplified calculation of expected life, as the Company does not currently have sufficient historical exercise data on which to base an estimate of expected term. Using this method, the expected term is determined using the average of the vesting period and the contractual life of the stock options granted.

Expected Volatility - Expected volatility is based on the Company’s historical stock volatility data over the expected term of the award.

Risk-Free Interest Rate - The Company bases the risk-free interest rate used in the Black-Scholes valuation method on the implied yield currently available on U.S. Treasury zero-coupon issues with an equivalent remaining term.

Forfeitures - The Company records stock-based compensation expense only for those awards that are expected to vest. The Company estimated a forfeiture rate of 5% for awards granted based on historical experience and future expectations of options vesting. The Company used this historical rate as our assumption in calculating future stock-based compensation expense.

1512

The Company recognized stock-based compensation expense of $~~64,483~~32,074 and $~~61,237~~63,458 for the three months ended ~~March 31,~~June 30, 2022 and 2021, respectively. The Company recognized stock-based compensation expense of $96,557 and $124,695 for the six months ended June 30, 2022 and 2021, respectively. The following table summarizes the effect of this stock-based compensation expense within each of the line items of our costs and expenses within our Consolidated Statements of Operations:

Schedule of Stock Based Compensation Expense

	For the Three Months Ended					For the Three Months Ended				For the Six Months Ended
	March 31,					June 30,				June 30,
	2022			2021		2022		2021		2022		2021
Cost of sales	$	4,349		$	5,053	$	2,161	$	5,107	$	6,510	$	10,160
Research and development		18,909			25,862		9,395		26,491		28,304		52,353
Selling and marketing		9,050			4,595		4,533		5,887		13,583		10,482
General and administrative		32,175	��		25,727		15,985		25,973		48,160		51,700
Total stock-based compensation expense	$	64,483		$	61,237	$	32,074	$	63,458	$	96,557	$	124,695

Fair Value of Financial Instruments

Due to their short maturities, the carrying amounts for cash and cash equivalents, accounts receivable, accounts payable, accrued expenses and debt approximate their fair value. The carrying amount of long-term debt approximates fair value due to interest rates that approximate prevailing market rates.

Fair Value Measurements

The Company follows the guidance of FASB ASC Topic 820, “Fair Value Measurements and Disclosures” (“ASC 820”) as it related to all financial assets and financial liabilities that are recognized or disclosed at fair value in the financial statements on a recurring basis.

The Company generally defines fair value as the price that would be received to sell an asset or paid to transfer a liability in an orderly transaction between market participants at the measurement date (exit price). The Company uses a three-tier fair value hierarchy, which classifies the inputs used in measuring fair values. These tiers include: Level 1, defined as observable inputs such as quoted prices for identical instruments in active markets; Level 2, defined as inputs other than quoted prices in active markets that are either directly or indirectly observable; and Level 3, defined as unobservable inputs in which little or no market data exists, therefore requiring the Company to develop its own assumptions. A slight change in an unobservable input like volatility could have a significant impact on fair value measurement.

Financial assets and liabilities are classified in their entirety based on the lowest level of input that is significant to the fair value measurement. The Company has determined that its financial assets are classified within Level 1 in the fair value hierarchy. The development of the unobservable inputs for Level 3 fair value measurements and fair value calculations are the responsibility of the Company’s management.

The following tables set forth the Company’s financial assets and liabilities that were accounted for at fair value on a recurring basis as of ~~March 31,~~June 30, 2022:

Schedule of Assets and Liabilities Measured at Fair Value on Recurring Basis

			Fair value measurements at March 31, 2022 using:								Fair value measurements at June 30, 2022 using:
	March 31, 2022		Quoted prices in active markets (Level 1)		Significant other observable inputs (Level 2)		Significant unobservable inputs (Level 3)		June 30, 2022		Quoted prices in active markets (Level 1)		Significant other observable inputs (Level 2)		Significant unobservable inputs (Level 3)
Equity Securities	$	79,114	$	79,114		-		-	$	60,604	$	60,604		-		-
Total Financial Assets	$	79,114	$	79,114	$	-	$	-	$	60,604	$	60,604	$	-	$	-

The following tables set forth the Company’s financial assets and liabilities that were accounted for at fair value on a recurring basis as of December 31, 2021:

			Fair value measurements at December 31, 2021 using:
	December 31, 2021		Quoted prices in active markets (Level 1)		Significant other observable inputs (Level 2)		Significant unobservable inputs (Level 3)
Equity Securities		59,976		59,976		-		-
Total Financial Assets	$	59,976	$	59,976	$	-	$	-

1713

4)Commitments and Contingencies

Operating Leases

The Company accounts for its leases under ASC 842. The Company has elected to apply the short-term lease exception to leases of one year or less.

Our corporate office is currently located at 14 Norfolk Avenue, South Easton, Massachusetts 02375. We are currently paying $6,950 per month, on a lease extension, signed on December 31, 2021, that expires December 31, 2022, for our corporate office. We expanded our space to include offices, warehouse and a loading dock on the first floor starting May 1, 2017 with a monthly rent increase already reflected in the current payments.

We extended our lease for our space in Medford, MA (the “Medford Lease”) from December 30, 2020 to December 30, 2023. The lease required monthly payments of $7,282 subject to annual cost of living increases. The lease shall be automatically extended for additional three years unless either party terminates at least six months prior to the expiration of the current lease term.

The Company accounted for the lease extension of our Medford Lease as a lease modification under ASC 842. At the effective date of modification, the Company recorded an adjustment to the right-of-use asset and lease liability in the amount of $221,432 based on the net present value of lease payments discounted using an estimated borrowing rate of 1212%%.

On August 9, 2021, we entered into an operating lease agreement for our warehouse space in Sparks, NV (the “Sparks Lease”) for the period from September 1, 2021 through September 30, 2026. The lease contains escalating payments during the lease period. The lease can be extended for an additional three years if the Company provides notice at least six months prior to the expiration of the current lease term.

The Company accounted for the Sparks Lease as an operating lease under ASC 842. Upon the commencement of the lease, the Company recorded a right-of-use asset and lease liability in the amount of $239,327 based on the net present value of lease payments discounted using an estimated borrowing rate of 1212%%.

Following is a schedule by years of future minimum rental payments required under operating leases with initial or remaining non-cancelable lease terms in excess of one year as of ~~March 31,~~June 30, 2022:

Schedule of Future Minimum Rental Payments Required Under Operating Leases


2022	$	188,210	$	115,551
2023		149,299		149,299
2024		64,393		64,393
2025		66,969		66,969
2026		51,778		51,778
Thereafter		-		-
Total	$	520,649	$	447,990

Battelle Memorial Institute

In December 2008, we entered into an exclusive patent license agreement with the Battelle Memorial Institute (“Battelle”). The licensed technology is the subject of a patent application filed by Battelle in 2008 and relates to a method and a system for improving the analysis of protein samples, including through an automated system utilizing pressure and a pre-selected agent to obtain a digested sample in a significantly shorter period of time than current methods, while maintaining the integrity of the sample throughout the preparatory process. In addition to royalty payments on net sales on “licensed products,” we are obligated to make minimum royalty payments for each year that we retain the rights outlined in the patent license agreement and we are required to have our first commercial sale of the licensed products within one year following the issuance of the patent covered by the licensed technology. After re-negotiating the terms of the contract in 2013, the minimum annual royalty was $1,200 in 2014 and $2,000 in 2015; the minimum royalties were $3,000 in 2016, $4,000 in 2017 and $5,000 in 2018 and each calendar year thereafter during the term of the agreement.

Target Discovery Inc.

In March 2010, we signed a strategic product licensing, manufacturing, co-marketing, and collaborative research and development agreement with Target Discovery Inc. (“TDI”), a related party. Under the terms of the agreement, we have been licensed by TDI to manufacture and sell ~~a highly~~an innovative line of chemicals used in the preparation of tissues for scientific analysis (“TDI reagents”). The TDI reagents were designed for use in combination with our pressure cycling technology. The companies believe that the combination of PCT and the TDI reagents can fill an existing need in life science research for an automated method for rapid extraction and recovery of intact, functional proteins associated with cell membranes in tissue samples. We did not incur any royalty obligation under this agreement in 2021 or 2020.

In April 2012, we signed a non-exclusive license agreement with TDI to grant the non-exclusive use of our pressure cycling technology. We executed an amendment to this agreement on October 1, 2016 wherein we agreed to pay a monthly fee of $1,400for the use of a lab bench, shared space and other utilities, and $2,000per day for technical support services as needed. The agreement requires TDI to pay the Company a minimum royalty fee of $60,000in 2021 and $60,000in 2022. For the ~~three~~six months ended ~~March 31,~~June 30, 2022 and ~~March 31,~~June 30, 2021, the Company reported $~~17,700~~ 49,400and $~~28,200~~34,400, respectively in TDI fees.

Severance and Change of Control Agreements

Each of Mr. Schumacher, and Drs. Ting, and Lazarev, executive officers of the Company, are entitled to receive a severance payment if terminated by us without cause. The severance benefits would include a payment in an amount equal to one year of such executive officer’s annualized base salary compensation plus accrued paid time off. Additionally, the officer will be entitled to receive medical and dental insurance coverage for one year following the date of termination.

Each of these executive officers, other than Mr. Schumacher, is entitled to receive a change of control payment in an amount equal to one year of such executive officer’s annualized base salary compensation, accrued paid time off, and medical and dental coverage, in the event of their termination upon a change of control of the Company. In the case of Mr. Schumacher, this payment would be equal to two years of annualized base salary compensation, accrued paid time off, and two years of medical and dental coverage. The severance payment is meant to induce the aforementioned executives to remain in the employ of the Company, in general; and particularly in the occurrence of a change in control, as a disincentive to the control change.

5)Convertible Debt and Other Debt

Convertible Debt

On various dates during the ~~three~~six months ended ~~March 31,~~June 30, 2022, the Company issued convertible notes for a total of $~~1,114,738~~2,624,738 which contained varied terms and conditions ~~as follows:~~including the following: a) 5-12 month maturity date; b) interest rates of 1212%%; c) convertible to the Company’s common stock at issuance at a fixed rate of $2.50 or at variable conversion rates upon the Company’s up-listing to NASDAQ or NYSE or an event of default. These notes were issued with either shares of common stock or warrants to purchase common stock that were fair valued at issuance date. The aggregate relative fair value of the shares of common stock and warrants issued with the notes of $~~229,916~~265,764 was recorded as a debt discount to be amortized over the term of the notes. We also evaluated the convertible notes for derivative liability treatment and determined that the notes did not qualify for derivative accounting treatment at ~~March 31,~~June 30, 2022.

1815

The specific terms of the convertible notes and outstanding balances as of ~~March 31,~~June 30, 2022 are listed in the tables below.

Schedule of Convertible Debts and Outstanding Balances

Inception Date	Term	Loan Amount		Outstanding balance with OID		Original Issue Discount (OID)		Interest Rate			Conversion Price			Deferred Finance Fees		Discount for conversion feature and warrants/shares		Term	Loan Amount		Outstanding balance with OID		Original Issue Discount (OID)		Interest Rate			Conversion Price			Deferred Finance Fees		Discount for conversion feature and warrants/shares

May 17, 2018 (1)(2)	12 months	$	380,000	$	98,544	$	15,200		8	%	$	2.50		$	15,200	$	332,407	12 months	$	380,000	$	98,544	$	15,200		8	%	$	2.50		$	15,200	$	332,407
January 3, 2019 (1)(4)	6 months	$	50,000	$	50,000	$	2,500		24	%	$	7.50		$	2,500	$	-	6 months	$	50,000	$	50,000	$	2,500		24	%	$	7.50		$	2,500	$	-
June 4, 2019 (1)(2)	9 months	$	500,000	$	302,484	$	-		8	%	$	2.50		$	40,500	$	70,631	9 months	$	500,000	$	302,484	$	-		8	%	$	2.50		$	40,500	$	70,631
July 19, 2019 (1) (2)	12 months	$	115,000	$	115,000	$	-		4	%	$	2.50		$	5,750	$	15,460	12 months	$	115,000	$	115,000	$	-		4	%	$	2.50		$	5,750	$	15,460
September 27,2019 (1) (2)	12 months	$	78,750	$	78,750	$	-		4	%	$	2.50		$	3,750	$	13,759	12 months	$	78,750	$	78,750	$	-		4	%	$	2.50		$	3,750	$	13,759
October 24, 2019 (1) (2)	12 months	$	78,750	$	78,750	$	-		4	%	$	2.50		$	3,750	$	-	12 months	$	78,750	$	78,750	$	-		4	%	$	2.50		$	3,750	$	-
November 15,2019 (1)	12 months	$	385,000	$	320,000	$	35,000		10	%	$	2.50		$	35,000	$	90,917	12 months	$	385,000	$	320,000	$	35,000		10	%	$	2.50		$	35,000	$	90,917
January 2,2020 (1)	12 months	$	330,000	$	330,000	$	30,000		10	%	$	2.50		$	30,000	$	91,606	12 months	$	330,000	$	330,000	$	30,000		10	%	$	2.50		$	30,000	$	91,606
January 24,2020 (1)	12 months	$	247,500	$	247,500	$	22,500		10	%	$	2.50		$	22,500	$	89,707	12 months	$	247,500	$	247,500	$	22,500		10	%	$	2.50		$	22,500	$	89,707
January 29, 2020 (1)	12 months	$	363,000	$	363,000	$	33,000		10	%	$	2.50		$	33,000	$	297,000	12 months	$	363,000	$	363,000	$	33,000		10	%	$	2.50		$	33,000	$	297,000
February 12, 2020 (1)	12 months	$	275,000	$	275,000	$	25,000		10	%	$	2.50		$	25,000	$	225,000	12 months	$	275,000	$	275,000	$	25,000		10	%	$	2.50		$	25,000	$	225,000
February 19,2020 (1)	12 months	$	165,000	$	165,000	$	15,000		10	%	$	2.50		$	15,000	$	135,000	12 months	$	165,000	$	165,000	$	15,000		10	%	$	2.50		$	15,000	$	135,000
March 11,2020 (1)	12 months	$	330,000	$	330,000	$	30,000		10	%	$	2.50		$	30,000	$	232,810	12 months	$	330,000	$	330,000	$	30,000		10	%	$	2.50		$	30,000	$	232,810
March 13, 2020 (1)	12 months	$	165,000	$	165,000	$	15,000		10	%	$	2.50		$	15,000	$	60,705	12 months	$	165,000	$	165,000	$	15,000		10	%	$	2.50		$	15,000	$	60,705
March 26, 2020 (1)	12 months	$	111,100	$	111,100	$	10,100		10	%	$	2.50		$	10,100	$	90,900	12 months	$	111,100	$	111,100	$	10,100		10	%	$	2.50		$	10,100	$	90,900
April 8, 2020 (1)	12 months	$	276,100	$	276,100	$	25,100		10	%	$	2.50		$	25,000	$	221,654	12 months	$	276,100	$	276,100	$	25,100		10	%	$	2.50		$	25,000	$	221,654
April 17,2020 (1)	12 months	$	143,750	$	143,750	$	18,750		10	%	$	2.50		$	-	$	96,208	12 months	$	143,750	$	143,750	$	18,750		10	%	$	2.50		$	-	$	96,208
April 30,2020 (1)	12 months	$	546,250	$	546,250	$	71,250		10	%	$	2.50		$	47,500	$	427,500	12 months	$	546,250	$	546,250	$	71,250		10	%	$	2.50		$	47,500	$	427,500
May 6, 2020 (1)	12 months	$	460,000	$	460,000	$	60,000		10	%	$	2.50		$	40,000	$	360,000	12 months	$	460,000	$	460,000	$	60,000		10	%	$	2.50		$	40,000	$	360,000
May 18,2020 (1)	12 months	$	546,250	$	221,250	$	46,250		10	%	$	2.50		$	35,500	$	439,500	12 months	$	546,250	$	221,250	$	46,250		10	%	$	2.50		$	35,500	$	439,500
June 2, 2020 (1)	12 months	$	902,750	$	652,750	$	92,750		10	%	$	2.50		$	58,900	$	708,500	12 months	$	902,750	$	652,750	$	92,750		10	%	$	2.50		$	58,900	$	708,500
June 12,2020 (1)	12 months	$	57,500	$	57,500	$	7,500		10	%	$	2.50		$	5,000	$	45,000	12 months	$	57,500	$	57,500	$	7,500		10	%	$	2.50		$	5,000	$	45,000
June 22, 2020 (1)	12 months	$	138,000	$	138,000	$	18,000		10	%	$	2.50		$	12,000	$	108,000	12 months	$	138,000	$	138,000	$	18,000		10	%	$	2.50		$	12,000	$	108,000
July 7, 2020 (1)	12 months	$	586,500	$	586,500	$	76,500		10	%	$	2.50		$	51,000	$	400,234	12 months	$	586,500	$	586,500	$	76,500		10	%	$	2.50		$	51,000	$	400,234
July 17, 2020 (1)	12 months	$	362,250	$	362,250	$	47,250		10	%	$	2.50		$	31,500	$	185,698	12 months	$	362,250	$	362,250	$	47,250		10	%	$	2.50		$	31,500	$	185,698
July 29, 2020 (1)	12 months	$	345,000	$	345,000	$	45,000		10	%	$	2.50		$	30,000	$	241,245	12 months	$	345,000	$	345,000	$	45,000		10	%	$	2.50		$	30,000	$	241,245
July 21, 2020 (1) (5)	12 months	$	115,000	$	115,000	$	15,000		10	%	$	2.50		$	10,000	$	24,875	12 months	$	115,000	$	115,000	$	15,000		10	%	$	2.50		$	10,000	$	24,875
August 14, 2020 (1)	12 months	$	762,450	$	462,450	$	69,450		10	%	$	2.50		$	66,300	$	580,124	12 months	$	762,450	$	462,450	$	69,450		10	%	$	2.50		$	66,300	$	580,124
September 10, 2020 (1)	12 months	$	391,000	$	391,000	$	51,000		10	%	$	2.50		$	34,000	$	231,043	12 months	$	391,000	$	391,000	$	51,000		10	%	$	2.50		$	34,000	$	231,043
September 21, 2020 (1) (5)	12 months	$	345,000	$	345,000	$	45,000		10	%	$	2.50		$	30,000	$	66,375	12 months	$	345,000	$	345,000	$	45,000		10	%	$	2.50		$	30,000	$	66,375
September 23, 2020 (1)	12 months	$	115,000	$	15,000	$	15,000		10	%	$	2.50		$	10,000	$	20,500	12 months	$	115,000	$	15,000	$	15,000		10	%	$	2.50		$	10,000	$	20,500
December 3, 2020 (1)	12 months	$	299,000	$	299,000	$	39,000		10	%	$	2.50		$	26,000	$	197,882	12 months	$	299,000	$	299,000	$	39,000		10	%	$	2.50		$	26,000	$	197,882
October 22, 2020 (1) (5)	12 months	$	115,000	$	115,000	$	15,000		10	%	$	2.50		$	10,000	$	18,875	12 months	$	115,000	$	115,000	$	15,000		10	%	$	2.50		$	10,000	$	18,875
February 17, 2021 (1)	12 months	$	230,000	$	230,000	$	30,000		10	%	$	2.50		$	20,000	$	180,000	12 months	$	230,000	$	230,000	$	30,000		10	%	$	2.50		$	20,000	$	180,000
March 23, 2021 (1)	12 months	$	55,000	$	55,000	$	5,000		10	%	$	2.50		$	-	$	36,431	12 months	$	55,000	$	55,000	$	5,000		10	%	$	2.50		$	-	$	36,431
May 6, 2021 (1)	12 months	$	402,500	$	402,500	$	52,500		10	%	$	2.50		$	35,000	$	312,551	12 months	$	402,500	$	402,500	$	52,500		10	%	$	2.50		$	35,000	$	312,551
June 17, 2021(1)	12 months	$	230,000	$	230,000	$	30,000		10	%	$	2.50		$	20,000	$	144,760	12 months	$	230,000	$	230,000	$	30,000		10	%	$	2.50		$	20,000	$	144,760
June 25, 2021(1)	12 months	$	977,500	$	977,500	$	127,500		10	%	$	2.50		$	-	$	773,802	12 months	$	977,500	$	977,500	$	127,500		10	%	$	2.50		$	-	$	773,802
June 3, 2021 (1)	6 months	$	50,000	$	50,000	$	1,500		12	%	$	2.50		$	-	$	7,948	6 months	$	50,000	$	50,000	$	1,500		12	%	$	2.50		$	-	$	7,948
March 1, 2022 (13)	6 months	$	700,000	$	700,000	$	84,000		12	%		(6	)	$	-	$	-	8 months	$	700,000	$	700,000	$	84,000		12	%		(6	)	$	-	$	-
July 3, 2021(1)	12 months	$	115,000	$	115,000	$	15,000		10	%	$	2.50		$	10,000	$	90,000	12 months	$	115,000	$	115,000	$	15,000		10	%	$	2.50		$	10,000	$	90,000
February 1, 2022 (14)	6 months	$	260,000	$	210,000	$	10,000		12	%	$	(7	)	$	2,000	$	-
February 1,2022 (1) (13)																		6 months	$	260,000	$	210,000	$	10,000		12	%		(7	)	$	2,000	$	-
February 4, 2022 (13)	8 months	$	500,000	$	500,000	$	30,000		12	%	$	(11	)	$	-	$	-	8 months	$	500,000	$	500,000	$	30,000		12	%		(11	)	$	-	$	-
July 6, 2021 (1)	6 months	$	130,000	$	130,000	$	10,000		12	%	$	2.50		$	-	$	-
July 15, 2021 (1)	6 months	$	100,000	$	45,000	$	5,000		12	%		(7	)	$	-	$	57,716
January 19, 2022 (14)	6 months	$	52,000	$	52,000	$	2,000		12	%	$	2.50		$	2,000	$	-
January 20, 2022 (3) (14)	6 months	$	352,188	$	352,188	$	45,938		(3	)		(8	)	$	-	$	-
January 20, 2022 (3) (14)	6 months	$	352,188	$	352,188	$	45,938		(3	)		(8	)	$	-	$	-
January 20, 2022 (3) (14)	6 months	$	140,875	$	140,875	$	18,375		(3	)		(8	)	$	-	$	-
May 13, 2022 (13)																		7 months	$	500,000	$	500,000	$	25,000		12	%		(11	)	$	-	$	-
January 19,2022 (1) (13)																		6 months	$	52,000	$	52,000	$	2,000		12	%	$	2.50		$	2,000	$	-
January 20,2022 (1) (3) (13)																		6 months	$	352,188	$	12,690	$	45,938		(3	)		(8	)	$	-	$	-
January 20,2022 (1) (3) (13)																		6 months	$	352,188	$	352,188	$	45,938		(3	)		(8	)	$	-	$	-
January 20,2022 (1) (3) (13)																		6 months	$	140,875	$	140,875	$	18,375		(3	)		(8	)	$	-	$	-
August 31, 2021	12 months	$	189,750	$	189,750	$	24,750		10	%		(9	)	$	16,500	$	148,500	12 months	$	189,750	$	189,750	$	24,750		10	%		(9	)	$	16,500	$	148,500
Sept. 8, 2021 (1)	8 months	$	78,000	$	78,000	$	3,000		12	%		(7	)	$	-	$	40,449
Sept. 10, 2021 (1)	8 months	$	100,000	$	100,000	$	4,000		12	%		(7	)	$	-	$	43,520
Sept. 15, 2021 (1)	6 months	$	250,000	$	250,000	$	12,500		12	%		(7	)	$	-	$	108,801
Sept. 16, 2021 (1)	6 months	$	250,000	$	250,000	$	12,500		12	%		(7	)	$	-	$	112,337
Sept. 24, 2021	8 months	$	125,000	$	125,000	$	6,250		12	%		(7	)	$	-	$	61,876
Sept. 15, 2021 (1)	6 months	$	250,000	$	250,000	$	37,500		12	%		(7	)	$	30,000	$	-
September 10, 2021 (1)																		8 months	$	100,000	$	100,000	$	4,000		12	%		(7	)	$	-	$	43,520
September 15, 2021 (1)																		6 months	$	250,000	$	250,000	$	12,500		12	%		(7	)	$	-	$	108,801
September 16, 2021 (1)																		6 months	$	250,000	$	250,000	$	12,500		12	%		(7	)	$	-	$	112,337
September 24, 2021 (1)																		8 months	$	125,000	$	125,000	$	6,250		12	%		(7	)	$	-	$	61,876
September 15, 2021 (1)																		6 months	$	250,000	$	250,000	$	37,500		12	%		(7	)	$	30,000	$	-
October 21, 2021 (5)	12 months	$	189,750	$	189,750	$	24,750		12	%	$	2.50		$	16,500	$	87,332	12 months	$	189,750	$	189,750	$	24,750		12	%	$	2.50		$	16,500	$	87,332
November 1, 2021 (5)	12 months	$	189,750	$	189,750	$	24,750		12	%	$	2.50		$	-	$	96,991	12 months	$	189,750	$	189,750	$	24,750		12	%	$	2.50		$	-	$	96,991
December 7, 2021	12 months	$	169,500	$	118,650	$	19,500		12	%		(10	)	$	3,750	$	-	12 months	$	169,500	$	67,800	$	19,500		12	%		(10	)	$	3,750	$	-
March 23,2022	8 months	$	56,500	$	56,500	$	6,500		12	%		(12	)	$	-	$	-
March 23, 2022																		8 months	$	56,500	$	35,312	$	6,500		12	%		(12	)	$	-	$	-
March 29, 2022	8 months	$	112,000	$	112,000	$	13,000		12	%		(12	)	$	-	$	-	8 months	$	112,000	$	67,144	$	13,000		12	%		(12	)	$	-	$	-
February 9,2022	12 months	$	88,987	$	80,112	$	10,237		12	%		(10	)	$	-	$	-
February 9, 2022																		12 months	$	88,987	$	53,487	$	10,237		12	%		(10	)	$	-	$	-
March 30, 2022	12 months	$	10,000	$	10,000	$	-		12	%		2.50		$	-	$	38,400	12 months	$	100,000	$	100,000	$	5,000		12	%	$	2.50		$	-	$	19,614
April 19, 2022																		12 months	$	95,000	$	95,000	$	-		12	%		(12	)	$	-	$	16,234
May 23, 2022																		8 months	$	950,000	$	950,000	$	57,000		12	%	$	2.50		$	16,165	$	-
May 8, 2022 (13) (14)																		8 months	$	65,000	$	65,000	$	3,000		12	%		(7	)	$	-	$	-
																					$	16,067,674	$	1,775,088							$	981,665	$	8,359,812
				$	15,103,691	$	1,703,088							$	965,500	$	8,460,529

	(1)	The Note is past due. The Company and the lender are negotiating in good faith to extend the loan.
	(2)	The Company and lenders have entered into Standstill and Forbearance Agreements (as described below).
	(3)	Note is secured by the assets of the Company’s subsidiary, PBI Agrochem, Inc. and interest rate is 40.9% OID.
	(4)	During the year ended December 31, 2020, the Company entered into a Rate Modification Agreement with this lender. In this agreement the lender agreed to reduce their interest rate and were granted the right to convert loans using a variable conversion price if more than one other variable rate lender converted at a variable rate.
	(5)	The Company has agreed to issue shares of its common stock to lenders if their notes are not repaid by a defined date.
	(6)	Loan is not convertible until 180 days from the date of issuance of the Note and following an Event of Default will be convertible at the lowest trading price of the 20 days prior to conversion. The loan is guaranteed by the Company’s Chief Executive Officer, but the lender may only enforce this guarantee after certain conditions have been met, specifically after (i) the occurrence of an Event of Default (as defined in the Note), (ii) the failure of the Company to cure the Default in 10 business days, and (iii) a failure by the Company to issue, or cause to be issued, shares of its common stock upon submission by the lender of a notice of conversion.
	(7)	Notes are convertible before maturity at $2.50 per share or mandatorily convertible when the Company up-lists to the NASDAQ at the lower of $2.50 or the up-list price.
	(8)	Notes can be converted at the lesser of $2.50 per share or 2525%% discount to the opening price of the Company’s first day of trading on either Nasdaq or NYSE. In addition, if the Company fails to pay the Note in cash on maturity date, the conversion price will be adjusted to the lesser of original conversion price or the product of the VWAP of the common stock for the 5 trading dates immediately prior to the maturity date multiplied by 0.75.
	(9)	Conversion price of this note is $2.50 and will be adjusted to, upon an Event of Default, the lower of (i) the conversion price or (ii) a 25% discount to the 5-day average VWAP of the stock prior to default. Additionally, if an up-list to a national exchange occurs while this note is outstanding, the conversion price shall be changed to the lower of (i) the conversion price or (ii) a 25% discount to the up-list price.
	(10)	Notes are convertible upon an Event of Default at 75% multiplied by the lowest trading price for the common stock during the five days prior to the conversion.
	(11)	Loans can be voluntarily converted before maturity at $2.50 per share. Lender retains the option upon an Up-list to convert at the lower of $2.50 or the 10% off Up-list price.
	(12)	Notes are convertible at $2.50 per share except that following an Event of Default the conversion price will be adjusted to 75% multiplied by the lowest trading price for the common stock during the five days prior to the conversion.
	(13)	During the ~~three~~six months ended ~~March 31,~~June 30, 2022, the Company extended ~~two~~nine loans totaling $~~475,000~~1,650,000 and increased the principal to $~~1,200,000~~2,872,251. The Company issued ~~60,000~~320,900 shares of common stock for these extensions and added principal.
	(14)	~~During the three months ended March 31, 2022, the Company extended five loans totaling $1,045,000~~Lender is a related party. ~~and increased the principal to~~ ~~1,107,251~~ ~~and issued~~ ~~149,500~~ ~~shares of common stock for the extensions.~~

As of ~~March 31,~~June 30, 2022, one lender holds approximately $9.4million of the $~~15.1~~ 16.1million convertible notes outstanding.

For the ~~three~~six months ended ~~March 31,~~June 30, 2022, the Company recognized amortization expense related to the debt discounts indicated above of $~~794,242~~1,363,151. The unamortized debt discounts as of ~~March 31,~~June 30, 2022 related to the convertible debentures and other convertible notes amounted to $~~813,478~~381,223.

Standstill and Forbearance Agreements

In recent years, the Company entered into Standstill and Forbearance Agreements with lenders who hold variable-rate convertible notes. Pursuant to these agreements the lenders agreed to not convert any portion of their notes into shares of common stock at a variable rate. The Company and two lenders ($673,528 outstanding principal at ~~March 31,~~June 30, 2022) are negotiating in good faith to resolve the remaining loans.

In connection to these agreements, the Company incurred interest, penalties, and fees of approximately $202,050 and $404,100in the three and six months ended ~~March 31, 2022.~~June 30, 2022, respectively.

Convertible Loan Modifications and Extinguishments

We refinanced certain convertible loans during the ~~three~~six months ended ~~March 31,~~June 30, 2022 at substantially the same terms for extensions ranging over a period of ~~three~~five to ~~six~~eight months. We amortized any remaining unamortized debt discount as of the modification date over the remaining, extended term of the new loans. We applied ASC 470 of modification accounting to the debt instruments which were modified during the quarter or those settled with new notes issued concurrently for the same amounts but different maturity dates. The terms such as the interest rate, prepayment penalties, and default rates will be the same over the new extensions. According to ASC 470, an exchange of debt instruments between or a modification of a debt instrument by a debtor and a creditor in a nontroubled debt situation is deemed to have been accomplished with debt instruments that are substantially different if the present value of the cash flows under the terms of the new debt instrument is at least 10 percent different from the present value of the remaining cash flows under the terms of the original instrument. If the terms of a debt instrument are changed or modified and the cash flow effect on a present value basis is less than 10 percent, the debt instruments are not considered to be substantially different and will be accounted for as modifications.

The cash flows of new debt exceeded 1010%% of the remaining cash flows of the original debt on several loans. During the ~~three~~six months ended ~~March 31,~~June 30, 2022 we recorded losses on extinguishment of liabilities of approximately $~~0.6~~0.8 million by calculating the difference of the fair value of the new debt and the carrying value of the old debt.

The following table provides a summary of the changes in convertible debt, net of unamortized discounts, during 2022:

Summary of Changes in Convertible Debt and Revolving Note Payable, Net of Unamortized Discounts

	2022			2022
Balance at January 1,	$	12,839,813		$	12,839,813
Early adoption of ASU 2020-06		473,027			473,027
Issuance of convertible debt, face value		1,114,738			2,624,738
Deferred financing cost		(308,738	)		(414,988	)
Debt discount from shares and warrants issued with debt		(229,916	)		(265,764	)
Payments		(324,350	)		(865,367	)
Conversion of debt into equity		(68,159	)		(68,159	)
Accretion of interest and amortization of debt discount to interest expense		794,242			1,363,151
Balance at March 31,		14,290,657
Balance at June 30,					15,686,451
Less: current portion		14,290,657			15,686,451
Convertible debt, long-term portion	$	–		$	–

Other Notes

On April 29, 2022, the Company borrowed $50,000 under a note from a lender which requires 52 weekly payments of $1,250. As of June 30, 2022, the loan has an outstanding balance of $42,308.

As of ~~March 31,~~June 30, 2022 the Company owes $691,500 on two notes to a private investor. During the ~~three~~six months ended ~~March 31,~~June 30, 2022, the Company issued 100,000 warrants (3 year term, $3.50 strike price) to the lender. The Company and the lender are negotiating in good faith to extend these loans.

On October 1, 2019, the Company and the holder of the $170,000 non-convertible loan issued in May 2017 agreed to extend the term of the loan to December 31, 2019. The Company agreed to issue 1,200 shares of its common stock per month while the note remains outstanding. The note will continue to earn 10% annual interest.The loan is currently past due and the Company and the investor are negotiating in good faith to extend the loan.

Merchant Agreements

We have signed various Merchant Agreements which are secured by second position rights to all customer receipts until the loan has been repaid in full and subject to interest rates of ~~10.8~~2.5-5.9% per month. As illustrated in the following table, under the terms of these agreements, we received the disclosed Purchase Price and agreed to repay the disclosed Purchase Amount, which is collected by the Merchant lenders at the disclosed Daily Payment Rate. The Company’s Chief Executive Officer (“Guarantor”) is guaranteeing that the Company will perform its obligations under the Agreement. In no circumstance will Guarantor be asked or obligated to repay or be liable for the payment of any amount paid by Buyer to Seller, including, but not limited to, thePurchasePrice.

The following table shows our Merchant Agreements as of ~~March 31,~~June 30, 2022:

Schedule of Merchant Agreements

Inception Date	Purchase Price		Purchased Amount		Outstanding Balance		Payment frequency	Payment Rate		Deferred Finance Fees
February 10, 2022	$	120,000	$	157,560	$	67,285	Weekly		7,878.00	$	1,200
												Purchase Price		Purchased Amount		Outstanding Balance		Payment frequency	Payment Rate		Deferred Finance Fees
June 28, 2022												$	250,000	$	337,250	$	248,295	Daily	$	2,595	$	-
June 15, 2022												$	150,000	$	197,850	$	141,280	Daily	$	1,522	$	-
May 11, 2022												$	225,000	$	308,250	$	181,467	Weekly	$	11,009	$	-
January 11, 2022	$	240,000	$	300,000	$	125,140	Weekly		11,112.00	$	-	$	240,000	$	300,000	$	2,456	Weekly	$	11,112	$	-
December 21, 2021	$	400,000	$	520,000	$	268,775	Weekly		11,305.00	$	6,000	$	400,000	$	520,000	$	161,449	Weekly	$	11,305	$	6,000
	$	760,000	$	977,560	$	461,200				$	7,200	$	1,265,000	$	1,663,350	$	734,947				$	6,000

The following table shows our Merchant Agreements as of December 31, 2021:

Inception Date	Purchase Price		Purchased Amount		Outstanding Balance		Payment frequency	Payment Rate		Deferred Finance Fees
December 21, 2021	$	400,000	$	520,000	$	390,120	Weekly		11,305.00	$	6,000
July 6, 2021		125,000		166,250		8,790	Daily		1,279.00		2,500
	$	525,000	$	686,250	$	398,910				$	8,500

We have accounted for the Merchant Agreements as loans under ASC 860 because while we provided rights to current and future receipts, we still had control over the receipts. The difference between the Purchase Amount and the Purchase Price is imputed interest that is recorded as interest expense when paid each day.

Related Party Notes

During the ~~three~~six months ended ~~March 31,~~June 30, 2022, we received short-term non-convertible loans of $~~$195,250~~ 516,450from related parties, which bear interest rates of ~~12%~~ 12%, have a 10% OID and are due upon demand. During this period we repaid $209,000 of these loans.

Long term debt

The Company entered into a COVID-19 government loan in 2020, the Economic Injury Disaster Loan (or “EIDL”). The Company’s EIDL loan, $150,000, accrues interest at 3.75%and requires monthly payments of $$731for principal and interest beginning in December 2022. The balance of the principal will be due in 30years. In connection with the EIDL loan the Company entered into a security agreement with the SBA, whereby the Company granted the SBA a security interest in all of the Company’s right, title and interest in all of the Company’s assets.

6)Stockholders’ Deficit

Preferred Stock

We are authorized to issue 1,000,000 shares of preferred stock with a par value of $0.01. Of the 1,000,000 shares of preferred stock:

	1)	20,000 shares have been designated as Series A Junior Participating Preferred Stock (“Junior A”)

	2)	313,960 shares have been designated as Series A Convertible Preferred Stock (“Series A”)

	3)	279,256 shares have been designated as Series B Convertible Preferred Stock (“Series B”)

	4)	88,098 shares have been designated as Series C Convertible Preferred Stock (“Series C”)

	5)	850 shares have been designated as Series D Convertible Preferred Stock (“Series D”)

	6)	500 shares have been designated as Series E Convertible Preferred Stock (“Series E”)

	7)	240,000 shares have been designated as Series G Convertible Preferred Stock (“Series G”)

	8)	10,000 shares have been designated as Series H Convertible Preferred Stock (“Series H”)

	9)	21 shares have been designated as Series H2 Convertible Preferred Stock (“Series H2”)

	10)	6,250 shares have been designated as Series J Convertible Preferred Stock (“Series J”)

	11)	15,000 shares have been designated as Series K Convertible Preferred Stock (“Series K”)

	12)	10,000 shares have been designated as Series AA Convertible Preferred Stock (“Series AA”)

As of ~~March 31,~~June 30, 2022, there were no shares of Junior A, and Series A, B, C and E issued and outstanding. See our Annual Report on Form 10-K for the year ended December 31, 2021 for the pertinent disclosures of preferred stock.

Stock Options and Warrants

At the Company’s December 30, 2021 Special Meeting, the shareholder’s approved the 2021 Equity Incentive Plan (the “2021 Plan”) pursuant to which 3,000,000 shares of our common stock were reserved for issuance upon exercise of stock options or other equity awards. Consistent with the Company’s existing 2013 Equity Incentive plan (the “2013 plan”), under the 2021 plan, we may award stock options, shares of common stock, and other equity interests in the Company to employees, officers, directors, consultants, and advisors, and to any other persons the Board of Directors deems appropriate. As of ~~March 31,~~June 30, 2022, options to acquire 1,307,822 shares were outstanding under these Plans.

As of ~~March 31,~~June 30, 2022, total unrecognized compensation cost related to the unvested stock-based awards was $~~108,471~~76,397, which is expected to be recognized over weighted average period of ~~0.82~~ 0.62~~year.~~ years. The aggregate intrinsic value associated with the options outstanding and exercisable, and the aggregate intrinsic value associated with the warrants outstanding and exercisable as of ~~March 31,~~June 30, 2022, based on the ~~March 31,~~June 30, 2022 closing stock price of $~~2.02~~1.74, was $~~1,707,483~~1,348,013.

The following table summarizes information concerning options and warrants outstanding and exercisable:

Schedule of Concerning Options and Warrants Outstanding and Exercisable

	Stock Options					Warrants										Stock Options					Warrants
	Weighted Average					Weighted Average										Weighted Average					Weighted Average
	Shares			price per share		Shares			price per share		Shares			Total Exercisable		Shares			price per share		Shares			price per share		Shares			Total Exercisable
Balance outstanding, December 31, 2021		1,333,101		$	0.72		16,207,108		$	3.50		17,540,209			17,308,567		1,333,101		$	0.72		16,207,108		$	3.50		17,540,209			17,308,567
Granted		-			-		230,000			3.50		230,000			-		-			-		230,000			3.50		230,000			-
Exercised		-			-		-			-		-			-		(25,279	)		0.69		-			-		(25,279	)		-
Expired/forfeited		(25,279	)		0.69		(86,670	)	$	3.50		(111,949	)		-		-			-		(149,172	)	$	3.50		(149,172	)		-
Balance outstanding, March 31, 2022		1,307,822		$	0.72		16,350,438		$	3.50		17,658,260			17,486,529
Balance outstanding, June 30, 2022																	1,307,822		$	0.72		16,287,936		$	3.50		17,595,758			17,475,939

As of ~~March 31,~~June 30, 2022, the 1,307,822options outstanding have a $0.72weighted average exercise price and ~~7.47~~ 7.22years weighted average remaining term. Of these options, ~~1,136,091~~ 1,188,003are currently exercisable.

Common Stock and Warrant Issuances

As profiled in the following table, for five loans we are obligated to issue common stock if not paid by defined dates.

Schedule of Loans Obligated to Issue Shares

	Loan Issuance	Loan		Percentage of Loan			Defined	Shares Issuable	Loan Issuance	Loan		Percentage of Loan			Defined	Shares Issuable
Loan	Date	Principal		Principal Issuable			Date	Frequency	Date	Principal		Principal Issuable			Date	Frequency

Loan 1	July 21, 2020	$	115,000		0.0435	%	September 30, 2020	Monthly	July 21, 2020	$	115,000		0.0435	%	September 30, 2020	Monthly
Loan 2	September 21, 2020	$	345,000		0.0362	%	November 16, 2020	Weekly	September 21, 2020	$	345,000		0.0362	%	November 16, 2020	Weekly
Loan 3	October 22, 2020	$	115,000		0.0652	%	December 1, 2020	Weekly	October 22, 2020	$	115,000		0.0652	%	December 1, 2020	Weekly
Loan 4	October 21, 2021	$	189,750		0.0435	%	January 2, 2022	Weekly	October 21, 2021	$	189,750		0.0435	%	January 2, 2022	Monthly
Loan 5	November 1, 2021	$	189,750		0.0435	%	January 2, 2022	Weekly	November 1, 2021	$	189,750		0.0435	%	January 2, 2022	Monthly

For the three-month and six-month period ended ~~March 31,2022~~June 30, 2022, the Company is obligated to issue ~~554,500~~224,500 and 782,600 shares of common stock, respectively, for the loans listed in the above table, but has not issued the shares. The Company and the lenders are negotiating in good faith to resolve these ~~loans and expect to reach a settlement in the coming month.~~loans. During the three-month and six-month period ended ~~March 31,~~June 30, 2022, the Company accrued $~~1,165,250~~388,515 and $1,553,765, respectively in interest expense for these obligations to issue common stock.

~~On various dates in~~During the ~~quarter~~six months ended ~~March 31,~~June 30, 2022, the Company issued a total of ~~1,073,610~~1,582,653 shares of restricted common stock to accredited investors and consultants. 140,200 of the shares with a fair value of $350,500 were issued for the conversion of debt and interest for common stock, ~~558,100~~782,600 of the shares with a fair value of $~~1,173,458~~1,561,973 were issued for interest paid-in-kind, ~~37,000~~77,000 of the shares with a fair value of $~~77,700~~145,500 were issued for services rendered, ~~31,810~~118,274 shares with a fair value of $~~64,256~~215,277 for dividends paid-in-kind, ~~92,000~~114,000 shares with a fair value of $~~142,480~~178,328 for new convertible debt issuances, ~~and~~ ~~214,500~~25,279 shares with a fair value of $~~472,900~~ 17,433 from a stock option exercise, 320,900 shares with a fair value of $664,203 for debt ~~extension.~~extension and shareholders converted 4 shares of Series AA Convertible Preferred Stock into 4,400 shares of common stock.

During the ~~three~~six months ended ~~March 31,~~June 30, 2022, we issued 100,000warrants (three-year term at a $3.50exercise price) to acquire common stock at a fair value of $87,436to a lender in conjunction with signing of new convertible loans. We also issued 30,000warrants (three-year term at a $3.50 exercise price) with a fair value of $39,761for services rendered and 100,000warrants (three-year term at a $3.50 exercise price) with a fair value of $132,537for debt extension.

During the ~~three~~six months ended ~~March 31,~~June 30, 2021, we issued ~~922,372~~1,642,982 shares of common stock with a fair value of approximately $~~2.0~~3.5 million to lenders for interest paid-in-kind, 112,400 shares with a fair value of $238,512 for services rendered, ~~47,200~~139,700 shares with a fair value of $~~118,000~~349,250 for conversions of debt principal and interest, ~~and~~ 21,411 shares for stock option exercises at(at an exercise price of $0.69 per ~~share.~~share), 56,067 shares with a fair value of $114,298 for dividends paid-in-kind and 120,000 shares with a fair value of $112,877 for Common Stock issued with debt. During this period, we also issued ~~521,200~~1,374,600 warrants (three to five-year term at a $3.50 to $5.00 exercise price) to acquire common stock at a fair value of $~~0.8~~1.7 million to lenders in conjunction with signing of new convertible loans and interest paid-in-kind.

7)Subsequent Events

~~After April~~From July 1, 2022 through August 8, 2022 the Company ~~issued~~ ~~22,000~~ ~~shares of common stock along with issuance of two convertible loans (totaling $200,000). Each loan carries a~~ ~~12%~~ ~~annual interest rate, a twelve-month term and is convertible at $2.50~~ ~~per share.~~

~~In this period, the Company also~~ borrowed $~~227,000~~203,000 from related parties ~~with~~ (due upon demand, 10% ~~original issue discount~~ OID and 12% ~~annual~~ interest ~~rate,~~) and ~~repaid $211,000~~ ~~of related party loans. The Company also~~ entered into a new merchant cash loan ~~arrangement (collecting~~agreement collecting $~~225,000~~180,000 ~~in return for contractual payments of~~(obligating the Company to repay $~~11,008~~1,868 per ~~week~~ day for ~~28 weeks)~~130 days). ~~During~~In this ~~period~~time the Company also issued ~~40,000~~112,500 shares of common stock to ~~professional services vendors and committed~~extend three convertible loans with approximately $618,000 principal for two to ~~issue~~ ~~20,000~~six months ~~shares in three months for service to be provided.~~.

ITEM 2. Management’s Discussion and Analysis of Financial Condition and Results of Operations

CAUTIONARY NOTE REGARDING FORWARD-LOOKING STATEMENTS

This Quarterly Report on Form 10-Q contains forward-looking statements within the meaning of Section 27A of the Securities Act of 1933, as amended (the “Securities Act”) and Section 21E of the Securities Exchange Act of 1934, as amended (the “Exchange Act”). In some cases, forward-looking statements are identified by terms such as “may,” “will,” “should,” “could,” “would,” “expects,” “plans,” “anticipates,” “believes,” “estimates,” “projects,” “predicts,” “potential” and similar expressions intended to identify forward-looking statements. Such statements include, without limitation, statements regarding:

	●	our need for, and our ability to raise, additional equity or debt financing on acceptable terms, if at all;
	●	our need to take additional cost reduction measures, cease operations or sell our operating assets, if we are unable to obtain sufficient additional financing;
	●	our belief that we will have sufficient liquidity to finance normal operations for the foreseeable future;
	●	the options we may pursue in light of our financial condition;
	●	the potential applications for Ultra Shear Technology (UST);
	●	the potential applications of the BaroFold high-pressure protein refolding and disaggregation technology
	●	the amount of cash necessary to operate our business;
	●	the anticipated uses of grant revenue and the potential for increased grant revenue in future periods;
	●	our plans and expectations with respect to our continued operations;
	●	the expected ~~increase in the~~ number of pressure cycling technology (“(“PCT”) and constant pressure (“(“CP”) based units that we believe will be installed and the expected ~~increase in~~ revenues from the sale of consumable products, extended service contracts, and biopharma contract services;
	●	our belief that PCT has achieved initial market acceptance in the mass spectrometry and other markets;
	●	the expected development and success of new instrument and consumables product offerings;
	●	the potential applications for our instrument and consumables product offerings;
	●	the expected expenses of, and benefits and results from, our research and development efforts;
	●	the expected benefits and results from our collaboration programs, strategic alliances and joint ventures;
	●	our expectation of obtaining additional research grants from the government in the future;
	●	our expectations of the results of our development activities funded by government research grants;
	●	the potential size of the market for biological sample preparation, biopharma contract services and Ultra Shear Technology;
	●	general economic conditions;
	●	the anticipated future financial performance and business operations of our company;
	●	our reasons for ~~focusing~~ resources expended in the market for genomic, proteomic, lipidomic and small molecule sample preparation;
	●	the importance of mass spectrometry as a laboratory tool;
	●	the advantages of PCT over other current technologies as a method of biological sample preparation and protein characterization in biomarker discovery, forensics, and histology, as well as for other applications;
	●	the capabilities and benefits of our PCT Sample Preparation System, consumables and other products;
	●	our belief that laboratory scientists will achieve results comparable with those reported to date by certain research scientists who have published or presented publicly on PCT and our other products and services;
	●	our ability to retain our core group of scientific, administrative and sales personnel; and
	●	our ability to expand our customer base in sample preparation and for other applications of PCT, as well as for our other products and services in both the BaroFold and Ultra Shear Technology areas.

These forward-looking statements are only predictions and involve known and unknown risks, uncertainties and other factors that may cause our actual results, levels of activity, performance or achievements to be materially different from any future results, levels of activity, performance or achievements, expressed or implied, by such forward-looking statements. Also, these forward-looking statements represent our estimates and assumptions only as of the date of this Quarterly Report on Form 10-Q. Except as otherwise required by law, we expressly disclaim any obligation or undertaking to release publicly any updates or revisions to any forward-looking statement contained in this Quarterly Report on Form 10-Q to reflect any change in our expectations or any change in events, conditions or circumstances on which any of our forward-looking statements are based. Factors that could cause or contribute to differences in our future financial and other results include those discussed in the risk factors set forth in Part I, Item 1A of our Annual Report on Form 10-K for the year ended December 31, 2021 and in this Report. We qualify all of our forward-looking statements by these cautionary statements.

OVERVIEW:

~~We are a leader in the development~~Pressure Biosciences, Inc. (“we”, “our”, “the Company”) develops and ~~sale of~~sells innovative, broadly enabling, high pressure-based platform ~~solutions~~technologies and related consumables for the worldwide life sciences, ~~industry.~~agriculture, food and beverage, and other key industries. Our solutions are based on the unique properties ~~of both~~and/or force generated from either constant (i.e., static) ~~and~~or alternating (i.e., ~~pressure cycling technology,~~Pressure Cycling Technology, or “PCT”) hydrostatic pressure. PCT is a patented enabling technology platform that uses alternating cycles of hydrostatic pressure between ambient and ultra-high levels to control bio-molecular interactions safely and reproducibly (e.g., cell lysis, biomolecule extraction). Our primary focus has historically been inIn the development of PCT-based products for biomarker and target discovery, drug design and development, biotherapeutics characterization and quality control, soil & plant biology, forensics, and counter-bioterror applications. Additionally,past five years, major new market opportunities have emerged in the use of our pressure-based ~~technology expertise in two new platform technology areas:~~technologies in: (1) the use of our recently acquired, patented technology from BaroFold, Inc. ~~for gently controlled disaggregation and refolding of biotherapeutic proteins~~ (the “BaroFold” technology) to allow entry into the bio-pharma contract services sector, and (2) the use of our recently-patented, scalable, high-efficiency, pressure-based Ultra Shear Technology (“UST”) platform to (i) create stable nanoemulsions of otherwise immiscible fluids (e.g., oils and water) for greatly improved cost-effectiveness, high bioavailability, safer and improved sensory experience in products spanning pharmaceuticals, nutraceuticals, cosmeceuticals, personal care products, agrochemicals, food/beverage and many industrial products and to (ii) prepare higher quality, homogenized, extended shelf-life or room temperature stable low-acid liquid foods that cannot be effectively preserved using existing non-thermal technologies. The Company’s initial growth and strong scientific reputation has been generated from PCT, a patented enabling technology platform that uses alternating cycles of hydrostatic pressure between ambient and ultra-high levels to safely and reproducibly control bio-molecular interactions (e.g., cell lysis, biomolecule extraction). While now focused predominantly on the enormous potential and markets for UST, and secondarily BaroFold, our historical concentration was in the development of PCT-based products for biomarker and target discovery, drug design and development, biotherapeutics characterization and quality control, soil & plant biology, forensics, and counter-bioterror applications.

Developments and Accomplishments:

We reported the following accomplishments during 2022:

	●	On August 5, PBI announces the doubling of growth projections for UST Nanoemulsion contracts following clear confirmation of unmet needs in exploding Cannabis Drinks Sector; Conferences on West Coast and Midwest illuminate rapid market expansion of Cannabis Beverages and critical role for UST Nanoemulsions to revolutionize Effective Precision Dosing and Delivery.

	●	On July 21, PBI announced the second contract for toll manufacturing of a CBD nano-emulsion product using PBI’s proprietary Ultra Shear Technology (UST).

	●	On June 29, PBI announced contracted production launch for an estimated $3 million of UST-processed, nano-emulsified CBD spray for oral use.

	●	On June 14, PBI announced that the UST Technology Platform is positioned for critical enabling role in global $41 Billion (2027) plant protein beverage market.
	●
	●	On May 4,, PBI announced the first nanoemulsions manufacturing agreement under recently released UST Early Access Program

	●	On April 27, PBI announced the availability of the Early Access Program for UST nanoemulsion processing platform.

	●	On April 5, PBI announces strong FY 2021 Financial and Operational Successes: FY 2021 revenue increased 64% over FY 2020, UST platform achieved critical goals – discussions ongoing with key leaders in multiple markets, and PCT/BaroFold/PBI Agrochem groups all reported important gains in FY ~~2022.~~2021.

	●	On March 17, PBI updates stakeholders with solid progress on key 2022 goals.

	●	On March 10, Emerging Technology Insider releases TechTalks video interview featuring PBI President Ric Schumacher, discussing OSU partnership, Food Industry Consortium, and commercialization of UST Platform. The interview can be found here: TechTalks Video

	●	On March 3, Ohio State announced they had installed & commissioned new pilot-scale UST processing equipment from PBI for the preparation of higher quality and safer liquid foods and beverages.

	●	On January 27, PBI’s UST Platform achieves key commercialization milestone with Installation and commissioning of production-scale BaroShear UST System at Ohio State to serve the food industry.

	●	On January 19, PBI participated in FORCE Family Office’s Webinar on Innovations and Advancements in the $4.6 Billion CBD Market. Click here to access the webinar: Innovations & Advancements in the CBD Market. 012021

2722

Results of Operations

The following disclosure compares the results of operations for the quarter ended ~~March 31,~~June 30, 2022 (“Q1Q2 2022”) with ~~March 31,~~June 30, 2021 (“Q1Q2 2021”). and compares the six months ended June 30,2022 with June 30, 2021.

Products and Services Revenue

We recognized total revenue of ~~$480,000~~$498,137 for Q1Q2 2022 compared to ~~$559,874~~$608,927 for Q1Q2 2021, a ~~14%~~18% decrease. For the year-to-date periods ending June 30,2022 and June 30,2021 we recognized revenue of $978,137 and $1,168,801, respectively, a 16% decrease.

The decline in revenue for the year-to-date periods was primarily attributable to a ~~$135,000~~$192,913 decrease in sales of PCT instrumentation and consumables, as Company resources were shifted primarily towards UST product and market development, which was offset by $83,292 of revenue from PBI Agrochem products (which were new in 2022).

Cost of Products and Services

The cost of products and services was ~~$314,363~~$302,141 for Q1Q2 2022 compared to ~~$226,275~~$286,660 for Q1Q2 2021. For the year-to-date periods ending June 30, 2022 and June 30, 2021 our cost of products and services was $616,504 and $512,935, respectively. Gross profit margin on products and services decreased to ~~35%~~37% in Q1the year-to-date period ended June 30, 2022 from ~~60%~~56% in Q1the same period ended June 30, 2021. ~~The~~This decrease ~~in Gross profit margin~~ was attributable to the sale of three instruments to a new foreign distribution relationship using long-term strategic pricing.

Research and Development

Research and development expenses were ~~$281,589~~$172,726 for Q1Q2 2022 compared to ~~$299,943~~$256,507 for Q1Q2 2021. For the year-to-date periods ending June 30,2022 and June 30,2021 these expenses were $454,315 and $556,450, respectively, an 18% decrease. The ~~reported decrease~~decreased expense was ~~6%.~~attributable to lower headcount in engineering.

Selling and Marketing

Selling and marketing expenses were ~~$66,462~~$129,434 for Q1Q2 2022 compared to ~~$93,328~~$92,813 for Q1Q2 2021. For the year-to-date periods ending June 30,2022 and June 30,2021 these expenses were $195,896 and $186,141, respectively, a 5% increase. The reported ~~decrease~~increase was primarily attributable to ~~reduced~~the hiring of new employees in sales and ~~marketing.~~marketing focused on UST market development.

General and Administrative

General and administrative expenses were ~~$903,885~~$795,466 for Q1Q2 2022 compared to ~~$1,015,430~~$619,286 for Q1Q2 2021, an increase of 28%. For the year-to-date periods ending June 30,2022 and June 30,2021 these expenses were $1,699,351 and $1,634,716, respectively, a ~~decrease of $111,545 or 11%. The decrease was primarily the result of a decrease in investor relations expenses.~~4% increase.

Operating Loss

Operating loss was ~~$1,086,299~~$901,630 for Q1Q2 2022 compared to ~~$1,075,102~~$646,339 for Q1Q2 2021, an increase of ~~$11,197, or 1%~~39%. For the year-to-date periods ending June 30,2022 and June 30,2021 the operating loss was $1,987,929 and $1,721,441 respectively, a 15% increase.

Interest Expense, net

Interest expense was ~~$2,579,161~~$1,835,589 for Q1Q2 2022 compared to ~~$4,668,064~~$3,526,141 for ~~Q1 2021.~~Q2 2021, a 48% decrease. For the year-to-date periods ending June 30,2022 and June 30,2021 these expenses were $4,414,750 and $8,194,205 respectively, a 46% decrease. The decrease was attributable to a decrease in amortization expense on unamortized discounts, lower interest expense for the issuance of common stock for interest paid-in-kind and a reduction in the Company’s interest expense incurred on standstill loans as part of these loans were settled in 2021.

Unrealized (loss) gain on investment in equity securities

Unrealized loss on investments in equity securities was $18,510 for Q2 2022 compared to an unrealized loss of $134,477 for Q2 2021. For the year-to-date period ending June 30,2022 the unrealized gain on investments in equity securities was ~~$19,138 for Q1 2022~~$628 as compared to ana $242,380 unrealized loss ~~of $107,903~~ for ~~Q1 2022.The~~the year-to-date period ending June 30,2021. The reported change was attributable to movement in the market price of the Company’s investment in Nexity.

Loss on extinguishment of liabilities

In connection with debt extensions and forgiveness, we recognized net losses of ~~$589,850~~$165,277 for Q1Q2 2022 compared to ~~$725,159~~$498,226 of losses for Q1Q2 2021. For the year-to-date periods ending June 30,2022 and June 30,2021 these losses were $755,127 and $1,223,385 respectively. The decline in losses was attributable to decreased extension and forgiveness activity.

Net loss attributable to common stockholders

Net loss attributable to common stockholders was ~~$4,671,834~~$3,348,046 ($~~0.48~~0.32 per share) for Q1Q2 2022 compared to ~~$7,038,686~~$5,149,342 ($~~1.45~~0.90 per share) for Q1Q2 2021. For the year-to-date periods ending June 30,2022 and June 30,2021 these losses were $8,019,880 ($0.80 per share) and $12,188,028 ($2.29 per share), respectively. The decrease in loss per share was attributable to the lower net loss attributable to common stockholders and to the increase in weighted shares outstanding.

Liquidity and Financial Condition

We have experienced negative cash flows from operations with respect to our pressure cycling technology business since our inception. As of ~~March 31,~~June 30, 2022, we did not have adequate working capital resources to satisfy our current liabilities and as a result, we have substantial doubt regarding our ability to continue as a going concern. As described in Notes 5 and 6 of the accompanying consolidated financial statements, we have been successful in raising debt and equity capital. We received ~~$1.4~~$4.0 million in net proceeds from loans in the ~~three~~six months ended ~~March 31,~~June 30, 2022. We have efforts in place to continue to raise cash through debt and equity offerings. (See Note 7 to the financial statements)

We will need substantial additional capital to fund our operations in future periods. If we are unable to obtain financing on acceptable terms, or at all, we will likely be required to cease our operations, pursue a plan to sell our operating assets, or otherwise modify our business strategy, which could materially harm our future business prospects.

Net cash used in operations for the ~~three~~six months ended ~~March 31,~~June 30, 2022 was ~~$756,282~~$1,998,192 as compared to ~~$1,237,015~~$2,090,727 for the ~~three~~six months ended ~~March 31,~~June 30, 2021.

Net cash used in investing activities for the ~~three~~six months ended ~~March 31,~~June 30, 2022 was ~~$1,135~~$4,890 compared to $3,962 in the ~~three~~six months ended ~~March 31,~~June 30, 2021.

Net cash provided by financing activities for the ~~three~~six months ended ~~March 31,~~June 30, 2022 was ~~$743,909~~$1,992,340 as compared to ~~$1,272,967~~$2,122,774 for the ~~three~~six months ended ~~March 31,~~June 30, 2021. The cash flows from financing activities in the ~~three~~six months ended ~~March 31,~~June 30, 2022 included ~~$1.4~~$4.0 million loan proceeds from convertible debt and other debt. In this period, cash flow from financing was reduced by debt payments of ~~$0.3~~$0.9 million on convertible debt, and ~~$0.3~~$1.1 million on other debt.

ITEM 3. QUANTITATIVE AND QUALITATIVE DISCLOSURES ABOUT MARKET RISK

This Item 3 is not applicable to us as a smaller reporting company and has been omitted.

ITEM 4. CONTROLS AND PROCEDURES

We maintain disclosure controls and procedures that are designed to ensure that information required to be disclosed in our Securities Exchange Act of 1934 filings are recorded, processed, summarized and reported within the time periods specified in the SEC’s rules and forms, and that such information is accumulated and communicated to our management, including our President and Chief Executive Officer (Principal Executive Officer) and Chief Financial Officer (Principal Financial Officer), as appropriate, to allow timely decisions regarding required disclosure. In designing and evaluating the disclosure controls and procedures, management recognized that controls and procedures, no matter how well designed and operated, can provide only reasonable assurance of achieving the desired control objectives, as ours are designed to do, and management was necessarily required to apply its judgment in evaluating the cost-benefit relationship of possible controls and procedures.

As of ~~March 31,~~June 30, 2022, we carried out an evaluation, under the supervision and with the participation of our management, including our Principal Executive Officer and Principal Financial Officer, of the effectiveness of the design and operation of our disclosure controls and procedures, as defined in Rules 13a-15(e) and 15d-15(e) under the Securities Exchange Act of 1934. Based upon that evaluation, our Principal Executive Officer and Principal Financial Officer concluded that our disclosure controls and procedures were not effective.

Our conclusion that our disclosure controls and procedures were not effective as of ~~March 31,~~June 30, 2022 is due to the continued presence of the material weaknesses in our internal control over financial reporting identified in our Annual Report on Form 10-K for the year ended December 31, 2021. These material weaknesses are the following:

	●	We identified a lack of sufficient segregation of duties. Specifically, this material weakness is such that the design over these areas relies primarily on detective controls and could be strengthened by adding preventative controls to properly safeguard Company assets.

	●	Management has identified a lack of sufficient personnel in the accounting function due to our limited resources with appropriate skills, training and experience to perform the review processes to ensure the complete and proper application of generally accepted accounting principles, particularly as it relates to valuation of warrants and other complex debt /equity transactions. Specifically, this material weakness resulted in audit adjustments to the annual consolidated financial statements and revisions to related disclosures, valuation of warrants and other equity transactions.

	●	Limited policies and procedures that cover recording and reporting of financial transactions.

	●	Lack of multiple levels of review over the financial reporting process

	We continue to plan to remediate those material weaknesses as follows:

	●	Improve the effectiveness of the accounting group by augmenting our existing resources with additional consultants or employees to assist in the analysis and recording of complex accounting transactions, and to simultaneously achieve desired organizational structuring for improved segregation of duties. We plan to mitigate this identified deficiency by hiring an independent consultant once we generate significantly more revenue or raise significant additional working capital.

	●	Improve expert review and achieve desired segregation procedures by strengthening cross approval of various functions including quarterly internal audit procedures where appropriate once we generate significantly more revenue or raise significantly more working capital.

During the period covered by this Report, we implemented and performed additional substantive procedures, such as supervisory review of work papers and consistent use of financial models used in equity valuations, to ensure our consolidated financial statements as of and for the three-month period ended ~~March 31,~~June 30, 2022, are fairly stated in all material respects in accordance with GAAP. We have not, however, been able to fully remediate the material weaknesses due to our limited financial resources. Our remediation efforts are largely dependent upon our securing additional financing to cover the costs of implementing the changes required. If we are unsuccessful in securing such funds, remediation efforts may be adversely affected in a material manner.

Except as described above, there have been no changes in our internal controls over financial reporting that occurred during the period ended ~~March 31,~~June 30, 2022 that have materially affected, or are reasonably likely to materially affect, our internal controls over financial reporting.

PART II. OTHER INFORMATION

Item 1. Legal Proceedings

We are not currently involved in any litigation that we believe could have a material adverse effect on our financial condition or results of operations. There is no action, suit, proceeding, inquiry or investigation before or by any court, public board, government agency, self-regulatory organization or body pending or, to the knowledge of the executive officers of our company or any of our subsidiaries, threatened against or affecting our company, our common stock, any of our subsidiaries or of our companies or our subsidiaries’ officers or directors in their capacities as such, in which an adverse decision could have a material adverse effect.

Item 1A. Risk Factors

Factors that could cause or contribute to differences in our future financial and operating results include those discussed in the risk factors set forth in Item 1 of our Annual Report on Form 10-K for the year ended December 31, 2021 and in this Item 1A. The risks described in our Form 10-K and this Report are not the only risks that we face. Additional risks not presently known to us or that we do not currently consider significant may also have an adverse effect on the Company. If any of the risks actually occur, our business, results of operations, cash flows or financial condition could suffer.

There have been no material changes to the risk factors set forth in Item 1A of our 10-K for the year ended December 31, 2021 other than the following:

We owe over $9 million to one lender with such loans secured by a security interest in all of our assets. If we default under our obligations pursuant to such loans, the lender could foreclose on all of our assets which could require us to cease our operations.

Through ~~March 31,~~June 30, 2022, we have issued Notes to the same holder such that the current gross amount owed to the holder is approximately $9.4 million. Our obligations under the Notes and the transaction documents relating to the Notes are secured by a security interest in all of our assets. As a result, if we default under our obligations under the Notes or the transaction documents, the holders of the Notes, acting through their appointed agent, could foreclose on their security interests and liquidate some or all of these assets, which could harm our business, financial condition and results of operations and could require us to reduce or cease operations. In addition, the pledge of these assets and other restrictions may limit our flexibility in raising capital for other purposes. Because all of our assets are pledged under these financing arrangements, our ability to incur additional secured indebtedness or to sell or dispose of assets to raise capital may be impaired, which could have an adverse effect on our financial flexibility.

The holders of our Common Stock could suffer substantial dilution due to our corporate financing practices.

The holders of our common stock could suffer substantial dilution due to our corporate financing practices which, in the past few years has included private placements. As of ~~March 31,~~June 30, 2022, we had ~~8,722,494~~8,997,037 shares outstanding. As of ~~March 31,~~June 30, 2022, if all of the outstanding shares of Series D Convertible Preferred Stock, Series G Convertible Preferred Stock, Series H Convertible Preferred Stock, Series H2 Convertible Preferred Stock, Series J Convertible Preferred Stock, Series K Convertible Preferred Stock and Series AA Convertible Preferred Stock were converted into shares of common stock and all outstanding options and warrants to purchase shares of common stock were exercised and all fixed rate convertible notes and debentures were converted, each as of ~~March 31,~~June 30, 2022, an additional ~~32,475,868~~32,842,695 shares of common stock would be issued and outstanding. The full cash exercise of the options and warrants would result in approximately ~~$58.1~~$57.9 million in cash proceeds to the Company. This additional issuance of shares of common stock would cause immediate and substantial dilution to our existing stockholders and could cause a significant reduction in the market price of our common stock.

Item 2. Unregistered Sales of Equity Securities and Use of Proceeds

Except where noted, all the securities discussed in this Part II, Item 2 were issued in reliance on the exemption under Section 4(a)(2) of the Securities Act.

~~On various dates in~~

During the ~~quarter~~six months ended ~~March 31,~~June 30, 2022, ~~the Company~~we issued ~~a total of 1,073,610~~1,582,653 shares of ~~restricted~~ common ~~stock to accredited investors and consultants.~~stock. We issued 77,000 shares with a fair value of $145,500 for services rendered, 140,200 ~~of the~~ shares with a fair value of $350,500 ~~were issued~~ for ~~the conversion~~conversions of debt principal and interest, 25,279 shares for ~~common~~ stock ~~558,100~~option exercises (at an exercise price of ~~the~~$0.69 per share), 118,274 shares with a fair value of ~~$1,173,458 were issued~~$215,277 for ~~interest~~dividends paid-in-kind ~~37,000 of the~~and 114,000 shares with a fair value $178,328 for new debt issuances. During this period, we also issued 782,600 shares of ~~$77,700 were issued for services rendered, 31,810 shares~~common stock with a fair value of ~~$64,256~~$1,561,973 for ~~dividends~~interest paid-in-kind, ~~92,000~~4,400 shares ~~with a fair value~~ of ~~$142,480~~common stock for ~~new~~the conversion of 4 shares of series AA convertible ~~debt issuances and 214,500 shares with a fair value of $472,900 for debt extension.~~

preferred stock. During the ~~three~~six months ended ~~March 31,~~June 30, 2022, we issued 100,000 warrants (three-year term at a $3.50 exercise price) to acquire common stock at a fair value of $87,436 to ~~lenders~~a lender in conjunction with signing of new convertible loans. We also issued 30,000 warrants (three-year term at a $3.50 exercise price) with a fair value of $39,761 for services rendered and 100,000 warrants (three-year term at a $3.50 exercise price) with a fair value of $132,537 for debt extension.

During the six months ended June 30, 2021, we issued 1,642,982 shares of common stock with a fair value of approximately $3.5 million to lenders for interest paid-in-kind, 112,400 shares with a fair value of $238,512 for services rendered, 139,700 shares with a fair value of $349,250 for conversions of debt principal and interest, 21,411 shares for stock option exercises (at an exercise price of $0.69 per share), 56,067 shares with a fair value of $114,298 for dividends paid-in-kind and 120,000 shares with a fair value $112,877 for Common stock issued with debt. During this period, we also issued 1,374,600 warrants (three to five year term at a $3.50 to $5.00 exercise price) to acquire common stock at a fair value of $1.7 million to lenders in conjunction with signing new convertible loans and interest paid-in-kind.

Item 3. Defaults upon Senior Securities

None.

Item 4. Mine Safety Disclosures

Not applicable.

Item 5. Other Information

~~None.~~

None.

Item 6. Exhibits

Exhibits

31.1*		Certification by the Principal Executive Officer of Registrant pursuant to Section 302 of the Sarbanes-Oxley Act of 2002 (Rule 13a-14(a) or Rule 15d-14(a))

31.2*		Certification by the Principal Financial Officer of Registrant pursuant to Section 302 of the Sarbanes-Oxley Act of 2002 (Rule 13a-14(a) or Rule 15d-14(a))

32.1**		Certification by the Principal Executive Officer pursuant to 18 U.S.C. 1350 as adopted pursuant to Section 906 of the Sarbanes-Oxley Act of 2002*

32.2**		Certification by the Principal Financial Officer pursuant to 18 U.S.C. 1350 as adopted pursuant to Section 906 of the Sarbanes-Oxley Act of 2002*

101.INS*		Inline XBRL Instance Document

101.SCH*		Inline XBRL Taxonomy Extension Schema Document

101.CAL*		Inline XBRL Taxonomy Extension Calculation Linkbase Document

101.DEF*		Inline XBRL Taxonomy Extension Definition Linkbase Document

101.LAB*		Inline XBRL Taxonomy Extension Label Linkbase Document

101.PRE*		Inline XBRL Taxonomy Extension Presentation Linkbase Document

104		Cover Page Interactive Data File (embedded within the Inline XBRL document)

* Filed herewith.

** In accordance with SEC Release 33-8238, Exhibits 32.1 and 32.2 are furnished and not filed.

SIGNATURES

Pursuant to the requirements of the Securities Exchange Act of 1934, the registrant caused this report to be signed on its behalf by the undersigned thereunto duly authorized.

	PRESSURE BIOSCIENCES, INC.

Date: ~~May 18,~~August 15, 2022	By:	/s/ Richard T. Schumacher
		Richard T. Schumacher
		President & Chief Executive Officer
		(Principal Executive Officer and Principal Financial Officer)

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