178 patents
Utility
Aldehyde Dehydrogenase Variants and Methods of Using Same
28 Dec 23
The invention provides polypeptides and encoding nucleic acids of aldehyde dehydrogenase variants.
Amit Shah, Joseph Warner
Filed: 28 Mar 23
Utility
Compositions and Multiplexed Systems for Coupled Cell-free Transcription-translation and Protein Synthesis and Methods for Using Them
14 Dec 23
In alternative embodiments, provided herein are transcription/translation (TX-TL) systems and methods of using them for use as rapid prototyping platforms for the synthesis, modification and identification of natural products (NPs), and natural product analogs (NPAs) and secondary metabolites, from biosynthetic gene cluster pipelines.
Stephanie CULLER, Ihsiung Brandon CHEN, Priti PHARKYA, Steve VAN DIEN, Nelson BARTON
Filed: 13 Mar 23
Utility
Microorganisms and methods for the biosynthesis of adipate, hexamethylenediamine and 6-aminocaproic acid
5 Dec 23
The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway.
Mark J. Burk, Anthony P. Burgard, Robin E. Osterhout, Priti Pharkya
Filed: 16 Nov 21
Utility
Prenyltransferase Variants and Methods for Production of Prenylated Aromatic Compounds
23 Nov 23
Described herein are prenyltransferases including non-natural variants thereof having at least one amino acid substitution as compared to its corresponding natural or unmodified prenyltransferases and that are capable of at least two-fold greater rate of formation of cannabinoids such as cannabigerolic acid, cannabigerovarinic acid, cannabigerorcinic acid, and cannabigerol, as compared to a wild type control.
Michael A. Noble, Kevin G. Hoff, Anna Lechner, Harish Nagarajan
Filed: 12 May 23
Utility
Recombinant microorganisms for the production of fatty amines
14 Nov 23
The disclosure relates to recombinant microorganisms for the production of fatty amines and derivatives thereof.
Stephen B. Del Cardayre, Louis G. Hom
Filed: 14 Dec 20
Utility
Microorganisms and methods for producing (3R)-hydroxybutyl (3R)-hydroxybutyrate
14 Nov 23
Provided herein are non-naturally occurring microbial organisms having a pathway for production of (3R)-hydroxybutyl (3R)-hydroxybutyrate, wherein the organism can further include a (R)-1,3-butanediol pathway, a (3R)-hydroxybutyrate pathway, a (3R)-hydroxybutyryl-CoA pathway, an acetoacetate pathway, an acetoacetyl-CoA pathway, a (3R)-hydroxybutyl-ACP pathway, or an acetoacetyl-ACP pathway.
Benjamin Adelstein, Robin E. Osterhout, Mark J. Burk
Filed: 23 May 14
Utility
Engineered Enzymes and Methods of Making and Using
2 Nov 23
The invention provides an engineered carboxylic acid reductase (CAR) enzyme, a nucleic acid encoding the CAR enzyme, and a non-naturally occurring microbial organism comprising an exogenous nucleic acid encoding the CAR, an engineered transaminase (TA) enzyme, and/or a hexamethylenediamine (HMD) transaminase (TA2) enzyme.
Amit Mahendra SHAH, Deqiang ZHANG, Joseph Roy WARNER, Benjamin Matthew GRIFFIN, Russell Scott KOMOR, Harish NAGARAJAN, Sankha GHATAK, Toby Howard RICHARDSON
Filed: 23 Apr 21
Utility
Flavin-dependent Oxidases Having Cannabinoid Synthase Activity
19 Oct 23
The disclosure relates to a non-natural flavin-dependent oxidase comprising at least one amino acid variation as compared to a wild type flavin-dependent oxidase, wherein the non-natural flavin-dependent oxidase does not comprise a disulfide bond, and wherein the non-natural flavin-dependent oxidase is capable of oxidative cyclization of a prenylated aromatic compound into a cannabinoid.
Andreas W. SCHIRMER, Deqiang ZHANG, Jamison Parker HUDDLESTON
Filed: 1 Sep 21
Utility
Microorganisms and Methods for Enhancing the Availability of Reducing Equivalents In the Presence of Methanol, and for Producing Adipate, 6-AMINOCAPROATE, Hexamethylenediamine or Caprolactam Related Thereto
19 Oct 23
Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol.
Anthony P. BURGARD, Robin E. OSTERHOUT, Stephen J. VAN DIEN, Cara Ann TRACEWELL, Priti PHARKYA, Stefan ANDRAE
Filed: 24 Jan 23
Utility
Microorganisms and Methods for Reducing By-products
14 Sep 23
The present disclosure provides microbial organisms having decreased production of unwanted by-products (e.g, pyruvate-, CO2—, TCA-derived by-products; acetate; ethanol; and/or, alanine) to enhance carbon flux through acetyl-CoA, which can increase production of acetyl-CoA derived compounds (e.g, 1,3-BDO, MMA, and (3R)-hydroxybutyl (3R)-hydroxybutyrate, or any other acetyl-CoA derived compounds), and products made from any of these compounds.
Harish Nagarajan, Gionata Scalcinati, Tae Hoon Yang
Filed: 28 Oct 20
Utility
Microorganisms and Methods for the Production of Butadiene Using Acetyl-coa
14 Sep 23
The invention provides non-naturally occurring microbial organisms containing butadiene or 2,4-pentadienoate pathways comprising at least one exogenous nucleic acid encoding a butadiene or 2,4-pentadienoate pathway enzyme expressed in a sufficient amount to produce butadiene or 2,4-pentadienoate.
Priti Pharkya, Anthony P. Burgard, Mark J. Burk
Filed: 20 May 22
Utility
Process for the production of epsilon caprolactam from 6-aminocaproic acid
12 Sep 23
A new process for the production of epsilon caprolactam (CPL) from 6-aminocaproic acid (6-ACA) can be obtained either from traditional petro chemical processes or can be obtained from biochemical processes.
Michele Cecchetto, Anacleto Dal Moro, Lauri Hannunpoika Suominen, Michael Japs
Filed: 23 Dec 19
Utility
Microorganisms and methods for enhancing the availability of reducing equivalents in the presence of methanol, and for producing adipate, 6-aminocaproate, hexamethylenediamine or caprolactam related thereto
12 Sep 23
Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol.
Anthony P. Burgard, Robin E. Osterhout, Stephen J. Van Dien, Cara Ann Tracewell, Priti Pharkya, Stefan Andrae
Filed: 16 Dec 13
Utility
Microorganisms for producing 1,3-butanediol and methods related thereto
25 Jul 23
Provided herein is a non-naturally occurring microbial organism having a 1,3-butanediol (1,3-BDO) pathway and comprising at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO.
Anthony P. Burgard, Mark J. Burk, Robin E. Osterhout, Jun Sun, Priti Pharkya
Filed: 5 Jun 20
Utility
Microorganisms and methods for the production of caprolactone
25 Jul 23
The invention provides non-naturally occurring microbial organisms containing caprolactone pathways having at least one exogenous nucleic acid encoding a butadiene pathway enzyme expressed in a sufficient amount to produce caprolactone.
Anthony P. Burgard, Robin E. Osterhout, Priti Pharkya, Mark J. Burk
Filed: 11 Dec 20
Utility
Method of producing and processing diamines to a diamine free base using a carbonate intermediate and an engineered microorganism
18 Jul 23
Provided is a method of producing and isolating a diamine produced by microbial fermentation that minimizes undesirable salt formation to provide a lower cost process.
Lauri H. Suominen, Connor J. Galleher, Michael Japs, Mark J. Burk, Cara Tracewell
Filed: 13 Jul 20
Utility
Prenyltransferase variants and methods for production of prenylated aromatic compounds
27 Jun 23
Described herein are prenyltransferases including non-natural variants thereof having at least one amino acid substitution as compared to its corresponding natural or unmodified prenyltransferases and that are capable of at least two-fold greater rate of formation of cannabinoids such as cannabigerolic acid, cannabigerovarinic acid, cannabigerorcinic acid, and cannabigerol, as compared to a wild type control.
Michael A. Noble, Kevin G. Hoff, Anna Lechner, Harish Nagarajan
Filed: 8 Mar 19
Utility
Omega-Hydroxylase-Related Fusion Polypeptide Variants With Improved Properties
25 May 23
The disclosure relates to omega-hydroxylase-related fusion polypeptides that result in improved omega-hydroxylated fatty acid derivative production when expressed in recombinant host cells.
Andreas SCHIRMER, Baolong ZHU, Emanuela POPOVA
Filed: 7 Jun 22
Utility
Alcohol Dehydrogenase Variants
25 May 23
Described herein are non-natural NAD+-dependent alcohol dehydrogenases (ADHs) capable of at least two fold greater conversion of methanol or ethanol to formaldehyde or acetaldehyde, respectively, as compared to its unmodified counterpart.
Stefan ANDRAE, Michael Patrick KUCHINSKAS, Jingyi LI, Harish NAGARAJAN, Priti PHARKYA
Filed: 8 Jul 22
Utility
3-HYDROXYBUTYRYL-COA Dehydrogenase Variants and Methods of Use
4 May 23
The present disclosure provides thiolases and polypeptide variants of 3-hydroxybutyryl-CoA dehydrogenase, nucleic acids encoding the same, vectors comprising the nucleic acids, and cells comprising the polypeptide variants and/or thiolase, the nucleic acids, and/or the vectors.
Kevin Hoff, Cara Ann Tracewell, Kui Chan, Michael Kuchinskas, Harish Nagarajan
Filed: 14 Jul 22