573 patents
Page 3 of 29
Utility
Methods for spatial analysis using rolling circle amplification and detection probes
28 Nov 23
Provided herein are methods of improving sensitivity of spatial detection of an analyte in a biological sample using splint oligonucleotides, circularized second strands, and rolling circle amplification.
Eswar Prasad Ramachandran Iyer, Yifeng Yin, Sultan Doganay Tuncer
Filed: 19 Nov 21
Utility
Systems and Methods for Inferring Cell Status
23 Nov 23
Systems and methods for inferring a status of a cell population are provided.
Michael Schnall-Levin, Rajiv Bharadwaj, Zahra Kamila Belhocine, Andrew D. Price, Yifeng Yin, Vijay Kumar Sreenivasa Gopalan, Zeljko Jovan Dzakula
Filed: 21 Apr 23
Utility
Engineered Thermophilic Reverse Transcriptase
23 Nov 23
The present disclosure relates generally to engineered nucleic acid processing enzymes and derivatives thereof, compositions and kits comprising the same; and methods of generating and using the same.
Shankar SHASTRY
Filed: 18 May 23
Utility
Rnase H Assisted In Situ Rolling Circle Amplification
23 Nov 23
The present disclosure relates in some aspects to methods for analyzing a target RNA in a biological sample, such as detection of a sequence of interest in an RNA.
Yufeng QIAN, Shankar SHASTRY
Filed: 28 Mar 23
Utility
Method of Identifying Circular Rna
23 Nov 23
The present disclosure relates in some aspects to methods and compositions for analyzing a biological sample, comprising performing rolling circle amplification of a circRNA in situ in a biological sample, thereby generating an RCA product comprising multiple complementary copies of the circRNA and detecting the RCA product in situ at a spatially localized position in the biological sample.
Justin COSTA
Filed: 18 May 23
Utility
Compositions and methods of making gene expression libraries
21 Nov 23
Provided herein are methods of detecting target nucleic acids and uses of the same.
Zachary Bent, Marlon Stoeckius
Filed: 29 Jan 21
Utility
Partition Processing Methods and Systems
16 Nov 23
The disclosure provides methods for separating and/or purifying one or more molecules released from one or more fluid compartments or partitions, such as one or more droplets.
Phillip Belgrader, Donald A. Masquelier
Filed: 3 Mar 23
Utility
Rna Templated Ligation
16 Nov 23
The present application provides methods for detecting a target nucleic acid molecule in a sample comprising contacting said sample with a ligatable probe comprising one or more parts and allowing said probe to hybridise to the target nucleic acid molecule, ligating any probe which has hybridised to the target nucleic acid molecule, amplifying the ligated probe, and detecting the amplification product, thereby to detect the target nucleic acid molecule, wherein said probes comprise at least one ribonucleotide at or near to a ligation site and/or wherein the probe or a probe part comprises an additional sequence 5′ to a target-specific binding site which is not hybridised to the target nucleic acid molecule upon hybridisation of the probe to the target nucleic acid molecule and forms a 5′ flap containing one or more nucleotides at its 3′ end that is cleaved prior to ligation, and methods of synthesising a DNA molecule with Phi29 DNA polymerase using a template nucleic acid molecule comprising at least one ribonucleotide.
Mats NILSSON, Malte KÜHNEMUND, Tomasz KRZYWKOWSKI
Filed: 19 Jul 23
Utility
Systems and Methods for Identifying Optimal D Gene Assignment And/orjunction Region Structure
16 Nov 23
A method is provided for identifying one or more D gene segment in a VDJ or VDDJ sequence.
David Benjamin Jaffe, Wyatt James McDonnell
Filed: 1 May 23
Utility
Systems and Methods for Visualization of Single-cell Resolution Characteristics
16 Nov 23
A dataset is obtained comprising data blocks, each representing a different characteristic, for a plurality of cells across a plurality of bins, each bin representing a different portion of a reference sequence.
Jeffrey Mellen, Kevin J. Wu, Vijay Kumar Sreenivasa Gopalan, Nicolaus Lance Hepler, Jasper Staab
Filed: 6 Feb 23
Utility
Methods, Compositions, and Kits for Blocking a Capture Probe on a Spatial Array
16 Nov 23
Provided herein are methods, compositions, and kits for determining a location of a target analyte in a biological sample that include the use of terminal deoxynucleotidyl transferase.
Jennifer Chew, David Michael Patterson
Filed: 27 Jul 23
Utility
Temperature Control Methods and Devices
9 Nov 23
The invention provides methods for rapid setting, adjusting, and controlling the temperature of a microfluidic device.
Michael John TIERNEY, Bill Kengli LIN, Rajiv VERMA, Lynna CHEN, Kevin GILMARTIN, Rajiv BHARADWAJ
Filed: 19 Jul 23
Utility
Spatially-tagged analyte capture agents for analyte multiplexing
7 Nov 23
Provided herein are methods for preparing biological samples for spatial proteomic analysis, methods of determining a location of a protein analyte in a biological sample, and methods of determining a location of a protein analyte and a nucleic acid analyte in a biological sample.
Cedric Uytingco, James Michael Chell, Marlon Stoeckius, Albert Dale Kim, Dulce Ovando Morales, Andrej Hartnett, Yifeng Yin, Jennifer Chew
Filed: 26 Jan 23
Utility
Systems and Methods for Liquid Deposition
2 Nov 23
Systems and methods for dispensing a liquid on a substrate are disclosed.
Yiran ZHANG, Siyuan XING, Denis PRISTINSKI
Filed: 5 Apr 23
Utility
Methods and Systems for Processing Polynucleotides
2 Nov 23
The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing.
Benjamin Hindson, Christopher Hindson, Michael Schnall-Levin, Kevin Ness, Mirna Jarosz, Serge Saxonov
Filed: 7 Jun 23
Utility
Sample Handling Apparatus and Image Registration Methods
2 Nov 23
A method for aligning a sample to an array is provided.
Augusto Manuel Tentori, Hanyoup Kim, Siyuan Xing, Felice Alessio Bava, Rajiv Bharadwaj, Cedric Uytingco, Erica Graziosa
Filed: 17 Sep 21
Utility
Methods and Systems for Sample Preparation and Analysis
26 Oct 23
The present disclosure provides methods and systems for sample preparation and/or analysis.
Zahra Kamila BELHOCINE, Josephine Lee, Francesca MESCHI, Luz MONTESCLAROS, Katherine PFEIFFER, Andrew D. PRICE, Jerald SAPIDA, David SUKOVICH, Tobias Daniel WHEELER, Yifeng YIN
Filed: 17 Jan 23
Utility
Spatial Analysis of Dna Methylation
26 Oct 23
Provided herein are methods of identifying a methylation status of an analyte in a biological sample.
Layla Katiraee, Michael Schnall-Levin, Christina Galonska, Marlon Stoeckius
Filed: 18 Apr 23
Utility
Spatial Transcriptomics for Antigen-receptors
26 Oct 23
Provided herein are methods, compositions, and kits for the detection of immune cell clonotypes and immune cell analytes within a biological sample.
Camilla Engblom, Kim Thrane, Jeffrey Mold, Jonas Frisen, Joakim Lundeberg, Qirong Lin
Filed: 10 May 23
Utility
Method for Transposase-mediated Spatial Tagging and Analyzing Genomic Dna In a Biological Sample
26 Oct 23
The present disclosure relates to materials and methods for spatially analyzing nucleic acids that have been fragmented with a transposase enzyme, alone or in combination with other types of analytes.
Michael Schnall-Levin, Michael Ybarra Lucero, Tarjei Sigurd Mikkelsen, Patrik Stahl, Jonas Frisen, Maja Marklund, Enric Llorens Bobadilla
Filed: 19 Sep 22